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Human knockouts?

William Wells

Author Affiliations

Genome Biology 2000, 1:spotlight-20000725-01  doi:10.1186/gb-spotlight-20000725-01

The electronic version of this article is the complete one and can be found online at:


Published:25 July 2000

© 2000 BioMed Central Ltd

Research news

Mobile group II intron RNAs insert directly into DNA target sites before being reverse-transcribed by an intron-encoded protein. Target site recognition involves base-pairing between the RNA and DNA, and interactions between flanking DNA and the intron-encoded protein. In the 21 July Science, Guo et al. use an Escherichia coli-based selection procedure and randomized intron sequences to derive group II introns capable of inserting into alternative target sites (Science 2000, 89:452-457). One of their test targets is the gene for the CCR5 chemokine receptor, inactivation of which is associated with HIV resistance. The targeting works in both bacterial and human cells transfected with a target plasmid, but experiments to test targeting to eukaryotic chromosomes are still in progress.

References

  1. Efficient integration of an intron RNA into double-stranded DNA by reverse splicing.

    PubMed Abstract OpenURL

  2. [http://www.sciencemag.org/] webcite

    Science magazine