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2D gels are not enough

William Wells

Author Affiliations

Genome Biology 2000, 1:spotlight-20000817-01  doi:10.1186/gb-spotlight-20000817-01

The electronic version of this article is the complete one and can be found online at:


Published:17 August 2000

© 2000 BioMed Central Ltd

Research news

A series of narrow-pH-range overloaded 2D gels, processed using sensitive silver-staining, yields enough spots that the gels could, theoretically, be surveying virtually all of the proteins of budding yeast. But in the August 15 Proceedings of the National Academy of Sciences, Gygi et al. report that proteomics proponents may have been fooling themselves (Proc Natl Acad Sci USA 2000, 97:9390-9395). Gygi et al. select a random region of one such gel and analyze all the visible spots. The genes corresponding to the identified proteins have a codon bias characteristic of high or medium abundance proteins. Many of these proteins are present in multiple spots, whereas many lower abundance proteins that would be expected to appear in the selected region (based on predicted molecular weight and pI) are not detected. This suggests that more sensitive chromatographic processes may be needed for complete proteomics analyses.

References

  1. [http://www.pnas.org/] webcite

    Proceedings of the National Academy of Sciences

  2. Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins.

    PubMed Abstract OpenURL