Table 2 |
|
|
Functional classification of cell-cycle-regulated transcripts found in Nb2 cells |
|
|
Category |
Transcript |
|
|
|
|
Cell cycle: cyclins and cell-cycle regulators |
|
|
Transcripts that have a cell-cycle-modulated homolog in yeast |
Cyclin E1 [78], peaks in G1 |
|
EGR-1, immediate-early gene (constitutive expression in Nb2 cells) |
|
|
Cdc5-like protein [79], peaks in M |
|
|
Cdk2, Cdk5 [78], peak in G1 |
|
|
Cell-cycle-modulated transcripts with a yeast homolog (not modulated) |
Cyclin B1, peaks in G2/M |
|
Cell-cycle-modulated transcripts with no yeast homolog |
Cyclin B2, peaks in G2 |
|
Cyclin D2 [8], peaks in G1 |
|
|
Cyclin D3 [8], peaks in G1 |
|
|
Nucleotide metabolism, DNA replication and repair |
Cdc21 homolog |
|
Spermidine/spermine N-acetyl transferase (SSAT) |
|
|
Ornithine decarboxylase (ODC) [80] |
|
|
S-adenosylmethionine decarboxylase [80] |
|
|
Prothymosin a |
|
|
PARP-2 (Unknown 6-45) |
|
|
Chromatin structure |
Histones H2A, H2B, peak in S in mammals and in yeast |
|
Cytoskeleton |
Myosin heavy chain |
|
Tubulin a, ß |
|
|
ß-actin [6] |
|
|
Clone 15 = rNUDC [81] |
|
|
FAK p125 |
|
|
Cell-surface antigens, adhesion molecules and signaling molecules |
|
|
(involved in apoptosis, survival and/or proliferation) |
|
|
Growth factor |
FGF-2 [82] |
|
Surface molecules |
Prolactin receptor Nb2 form [16] |
|
T-cell receptor ? chain [17] |
|
|
T-cell receptor a chain [17] |
|
|
GnRH receptor [83] |
|
|
Glucocorticoid receptor |
|
|
Galectin-8 |
|
|
Leukocyte membrane glycoprotein, CD45 |
|
|
Vitamin D3 receptor |
|
|
Thromboxane A2 receptor |
|
|
ß2-microglobulin |
|
|
Cytoplasmic and/or nuclear signaling molecules |
p38 Map kinase [21] |
|
Pim-1 [9] |
|
|
Gfi-1 [10] |
|
|
Stathmin [84] |
|
|
P13 kinase p110 a |
|
|
Phospholipase C?1 |
|
|
14-3-3 ? and e |
|
|
Bax |
|
|
p53 |
|
|
RexB/NSP |
|
|
Phosphatidylinositol transfer protein |
|
|
a 4 phosphoprotein [79] |
|
|
Transcription factors |
IRF-1 [7] |
|
c-Myc [18] |
|
|
c-Fos (not in Nb2 cells [18]) |
|
|
MRG1-related protein |
|
|
E2F-1 [78] |
|
|
Zfx |
|
|
Heat shock, stress response and chaperones |
Cyclophilin (B) |
|
TCP-1 e and ? |
|
|
Hsp70 (not expressed in Nb2 cells) |
|
|
Hsp70-like = Nb29 [41] |
|
|
Hsp27 |
|
|
Hsp86 |
|
|
ß-actin |
|
|
a2-tubulin |
|
|
Rdnuc (Golgi-associated protein) [11] |
|
|
Myosin heavy chain |
|
|
Focal adhesion kinase (FAK) |
|
|
Metabolism (energy) |
Phosphoglycerate kinase |
|
Enolase a |
|
|
Aldehyde dehydrogenase |
|
|
ATP synthase ß subunit |
|
|
Protein and RNA synthesis, modifications and degradation |
|
|
Ribosomal proteins |
L3, L12, L13A, new ribosomal protein L15 type (unknown 4-27) |
|
S8, S13 |
|
|
Glycosylation factor |
Itm1 |
|
Elongation factor |
EF-2 [77] |
|
Inter-compartment transport and trafficking |
CRM-1 = exportin 1 |
|
Sec-22 |
|
|
Glycine transporter |
|
|
Unknown 4-58 (SH3, Rab GAP, TBC domain: putative nuclear pore protein) |
|
|
Unknown function(s) |
FGF-responsive Non/p54nrb |
|
Unknowns T22, T34, 4-2, 4-4, 4-11, 4-15, 4-20, 4-49, 4-59, BO1 |
|
|
Unknowns 6-2, 6-3, 6-4, 6-9, 6-10, 6-12 |
|
|
|
|
|
Bole-Feysot et al. Genome Biology 2000 1:research0008.1 doi:10.1186/gb-2000-1-4-research0008 |
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