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Downsizing the Paramecium genome

Jonathan B Weitzman

Author Affiliations

Genome Biology 2000, 1:spotlight-20001109-02  doi:10.1186/gb-spotlight-20001109-02


The electronic version of this article is the complete one and can be found online at:


Published:9 November 2000

© 2000 BioMed Central Ltd

Research news

The elimination of specific DNA elements is a common feature in the formation of a transcriptionally active macronucleus during the sexual reproduction of ciliated protozoa. The micronuclear DNA of Paramecium tetraurelia contains some 50,000 internal eliminated sequences (IES), each of which is flanked by TA dinucleotide repeats and inverted sequences. In the November Molecular and Cellular Biology Ku et al. describe an in vivo method to analyse IES excision (Mol Cell Biol 2000, 20:8390-8396). They injected DNA containing a 28 base pair IES and a drug-resistance gene into mated ciliate cells. They selected for transformants and assayed for excision by simple PCR amplification. Analysis of various deletions showed that flanking sequences are essential for excision, whereas mutations within the IES had no effect. This study reveals similarities between Paramecium and DNA excision events in Tetrahymena.

References

  1. Genome downsizing during ciliate development: nuclear division of labor through chromosome restructuring.

    PubMed Abstract | Publisher Full Text OpenURL

  2. [http://intl-mcb.asm.org] webcite

    Molecular and Cellular Biology

  3. Programmed DNA deletions in Tetrahymena: mechanisms and implications.

    PubMed Abstract | Publisher Full Text OpenURL