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FLiP-ing insulators

Jonathan Weitzman

Author Affiliations

Genome Biology 2000, 1:spotlight-20001122-01  doi:10.1186/gb-spotlight-20001122-01


The electronic version of this article is the complete one and can be found online at:


Published:22 November 2000

© 2000 BioMed Central Ltd

Research news

Insulators define independent genomic regions that are protected from the influence of distant regulatory sequences. In the 1 November EMBO Journal, Parnell and Geyer describe a novel application of the FLP recombinase to investigate the properties of two Drosophila insulators, called gypsy and scs (EMBO J 2000, 19:5864-5874). Neither insulator affected FLP recombination and protein-protein interactions at adjacent recombination sites, suggesting that gypsy and scs do not act by general inhibition or chromatin structure rearrangement. The gypsy insulator could, however, block enhancer activity on extrachromosomal episomes created by FLP recombination, whereas the scs insulator acted as a gene silencer. So gypsy and scs appear to insulate genomic domains by two different enhancer-blocking mechanisms, which involve specific interference rather than global repression.

References

  1. [http://www.emboj.org] webcite

    EMBO Journal

  2. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome.

    PubMed Abstract | Publisher Full Text OpenURL

  3. DNA position-specific repression of transcription by a Drosophila zinc finger protein.

    PubMed Abstract OpenURL

  4. A group of scs elements function as domain boundaries in an enhancer-blocking assay.

    PubMed Abstract OpenURL