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Two for the price of one

Jonathan Weitzman

Author Affiliations

Genome Biology 2000, 1:spotlight-20001124-01  doi:10.1186/gb-spotlight-20001124-01


The electronic version of this article is the complete one and can be found online at:


Published:24 November 2000

© 2000 BioMed Central Ltd

Research news

The nuclear-encoded RNA polymerase RpoT;2 from Arabidopsis thaliana differs from the polymerases that transcribe the plant's nuclear genes and resembles RNA polymerases from bacteriophages. In the 15 November EMBO Reports, Hedtke et al. describe the use of GFP (green fluorescent protein) fusion proteins to examine the subcellular localization of RpoT;2 (EMBO Reports 2000, 1:435-440). The RpoT;2 transit peptide targeted GFP fusion proteins to both mitochondrial and chloroplast compartments in tobacco protoplasts and transgenic Arabidopsis plants. RpoT;2 may therefore be able to transcribe genes from two different genomes. This is in contrast to two other phage-type RNA polymerases from Arabidopsis, which are exclusively targeted to either mitochondria (RpoT;1) or chloroplasts (RpoT;3).

References

  1. [http://www.embo-reports.oupjournals.org] webcite

    EMBO Reports

  2. [http://pantheon.cis.yale.edu/~wfm5/gfp_gateway.html] webcite

    GFP applications page