|
Induction methods for RNAi by feeding |
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| Non-Ind |
Ind (1) |
Ind (2) |
Ind (3) |
Ind (4) |
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| Test gene |
n |
% Phe |
n |
% Phe |
n |
% Phe |
n |
% Phe |
n |
% Phe |
|
|
||||||||||
| gpb-1 |
546 |
0 |
530 |
100 |
309 |
84 |
442 |
97 |
346 |
0 |
| unc-22 |
422 |
0 |
255 |
99 |
179 |
80 |
ND |
ND |
ND |
ND |
| par-1 |
ND |
ND |
313 |
100 |
263 |
100 |
ND |
ND |
ND |
ND |
| par-3 |
ND |
ND |
391 |
96 |
325 |
11 |
ND |
ND |
ND |
ND |
|
Four different methods were compared to determine optimal induction conditions for RNAi; non-induced (Non-Ind) bacteria were also included for comparison. Induction conditions (Ind) were as follows: (1) Bacteria were induced on plates with IPTG at room temperature overnight; (2) bacteria were induced in culture at 37°C for 2 h; (3) bacteria were induced on plates with IPTG at 37°C overnight; (4) bacteria were induced in culture at 37°C overnight (see the Materials and methods section for detailed protocols). gpb-1, par-1 and par-3 were scored for percentage of dead embryos, unc-22 was scored for percentage of worms with an uncoordinated phenotype. Data shown represent the progeny of three fed worms. ND, not done; n is the number of worms or embryos scored; %Phe, percentage of worms or embryos with phenotype. | ||||||||||
Kamath et al. Genome Biology 2000 2:research0002.1 doi:10.1186/gb-2000-2-1-research0002 |
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