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Sensitivity of RNAi by feeding versus injection |
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| Gene(s) |
Feeding phenotype |
Injection phenotype |
Gene information |
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| K04G2.8a |
Unc, Bmd, Lvl |
Emb |
apr-1 (APC-related) |
| F18C12.2a |
Emb, Unc, Mlt, Bmd |
Emb, Unc, Mlt, Lvl |
DNA-J domain |
| ZK265.5,6* |
Gro |
Gro |
G-protein-coupled receptor/unknown function |
| T01G9.4 |
Emb, Gro, Clr |
Emb, Gro |
kup-2 (unknown function) |
| T01G9.5 |
Emb |
Emb |
mei-1 (meiotic spindle formation) |
| T01G9.6a |
Emb, Gro, Pvl |
Emb |
kin-10 (CKII-beta subunit) |
| F52B5.6 |
Emb, Ste |
Emb, Ste |
Ribosomal protein L25 |
| T19A6.2a |
Gro |
Gro |
Ynr053p-like protein |
| D1081.2 |
Unc, Prz |
Unc |
MADS domain |
| D1081.8 |
Emb |
Emb |
Myb-like DNA-binding domain |
| K02B12.1 |
Unc, Mlt |
Gro, Mlt, Lvl |
ceh-6 (POU homeodomain protein) |
| K02B12.3 |
Ste, Gro |
Ste |
WD domains |
| K02B12.8 |
Him |
None |
Unknown function |
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From chromosome 1, 86 random genes from consecutive cosmids were selected to test the sensitivity of feeding versus injection for detecting RNAi phenotypes. Of these, 13 gave a phenotype by either method, 13 by feeding and 12 by injection; 11 of these genes have no previously described mutant or RNAi phenotype. Genes were determined to have a given phenotype if at least 10% of progeny had that phenotype, except for sterility, which required an average brood size of less than ten, and the Him phenotype, which required at least two out of three fed worms to have >5% male progeny. Bmd, body morphology defect; Clr, clear; Emb, embryonic lethal; Gro, slow growth; Him, high incidence of males; Lvl, larval lethal (death at any larval stage); Mlt, molting defect (old cuticle remains attached); Prz, paralyzed; Pvl, protruding vulva; Ste, sterile; Unc, uncoordinated. *The fragment used overlaps two predicted genes: ZK265.5 and ZK265.6. | |||
Kamath et al. Genome Biology 2000 2:research0002.1 doi:10.1186/gb-2000-2-1-research0002 |
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