Table 4

Sensitivity of RNAi by feeding versus injection

Gene(s)

Feeding phenotype

Injection phenotype

Gene information

K04G2.8a

Unc, Bmd, Lvl

Emb

apr-1 (APC-related)

F18C12.2a

Emb, Unc, Mlt, Bmd

Emb, Unc, Mlt, Lvl

DNA-J domain

ZK265.5,6^*

Gro

Gro

G-protein-coupled receptor/unknown function

T01G9.4

Emb, Gro, Clr

Emb, Gro

kup-2 (unknown function)

T01G9.5

Emb

Emb

mei-1 (meiotic spindle formation)

T01G9.6a

Emb, Gro, Pvl

Emb

kin-10 (CKII-beta subunit)

F52B5.6

Emb, Ste

Emb, Ste

Ribosomal protein L25

T19A6.2a

Gro

Gro

Ynr053p-like protein

D1081.2

Unc, Prz

Unc

MADS domain

D1081.8

Emb

Emb

Myb-like DNA-binding domain

K02B12.1

Unc, Mlt

Gro, Mlt, Lvl

ceh-6 (POU homeodomain protein)

K02B12.3

Ste, Gro

Ste

WD domains

K02B12.8

Him

None

Unknown function

From chromosome 1, 86 random genes from consecutive cosmids were selected to test the sensitivity of feeding versus injection for detecting RNAi phenotypes. Of these, 13 gave a phenotype by either method, 13 by feeding and 12 by injection; 11 of these genes have no previously described mutant or RNAi phenotype. Genes were determined to have a given phenotype if at least 10% of progeny had that phenotype, except for sterility, which required an average brood size of less than ten, and the Him phenotype, which required at least two out of three fed worms to have >5% male progeny. Bmd, body morphology defect; Clr, clear; Emb, embryonic lethal; Gro, slow growth; Him, high incidence of males; Lvl, larval lethal (death at any larval stage); Mlt, molting defect (old cuticle remains attached); Prz, paralyzed; Pvl, protruding vulva; Ste, sterile; Unc, uncoordinated. ^*The fragment used overlaps two predicted genes: ZK265.5 and ZK265.6.

Kamath et al. Genome Biology 2000 2:research0002.1   doi:10.1186/gb-2000-2-1-research0002