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Transcriptional targets

Jonathan B Weitzman

Author Affiliations

Genome Biology 2001, 2:spotlight-20010130-01  doi:10.1186/gb-spotlight-20010130-01


The electronic version of this article is the complete one and can be found online at:


Published:30 January 2001

© 2001 BioMed Central Ltd

Research news

In the January 25 Nature, Iyer et al. describe an elegant technique to identify transcriptional target genes throughout the genome (Nature 2001, 409:533-538). They combined chromatin immunoprecipitation (ChIP) with microarray analysis (DNA chips) to probe individual protein-genome interactions. The technique involved cross-linking, immunoprecipitation, PCR amplification and fluorescent labeling, followed by hybridization to microarrarys containing genomic DNA. They searched the yeast genome for intergenic regions that contained functional binding sites for the cell cycle transcription factors SBF (Swi6/Swi4) and MBF (Swi6/Mbp1). They identified 183 SBF-regulated genes, many of which are involved in budding or cell wall biogenesis. The 98 potential MBF target genes include those implicated in DNA replication, recombination and repair. And 43 loci appear to be targets of both factors. This is a powerful technique for identifying common and distinct targets of related transcription factors.

References

  1. [http://www.nature.com] webcite

    Nature

  2. [http://www.microarrays.org] webcite

    Microarrays.org

  3. [http://genome-www.stanford.edu/Saccharomyces/] webcite

    Saccharomyces genome database

  4. [http://genome-www.Stanford.edu/chromatinip] webcite

    Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF