Ultraviolet sensitivity

The systematic deletion of all yeast ORFs, in the Saccharomyces Genome Deletion Project, provides a powerful resource for large-scale 'parallel deletion analysis'. In the October 23 Proceedings of the National Academy of Sciences, Geoff Birrell and colleagues at Stanford University School of Medicine describe a screen for sensitivity to a genome-damaging agent (Proc Natl Acad Sci USA 2001, 98:12608-12613). They screened pools of 4,627 deletion strains for killing by ultraviolet (UV) irradiation and used oligonucleotide arrays to follow radiation sensitivity. They identified 25 known UV-sensitive deletions and found three new genes not previously linked with UV sensitivity. Two of these, CaSm and AF9, have human orthologs implicated in cancer, demonstrating how such a powerful approach can provide important insights into the mechanism of cytotoxicity by genotoxic agents.

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   Saccharomyces Genome Deletion Project

2. Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy.

   PubMed Abstract

3. [http://www.pnas.org] webcite

   Proceedings of the National Academy of Sciences

4. [http://www-med.stanford.edu] webcite

   Stanford University School of Medicine

5. The Saccharomyces repair genes at the end of the century.

   PubMed Abstract | Publisher Full Text