Email updates

Keep up to date with the latest news and content from Genome Biology and BioMed Central.

Open Access Highly Accessed Research

Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions

Brian B Haab12, Maitreya J Dunham1 and Patrick O Brown1*

Author Affiliations

1 Department of Biochemistry and Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA

2 Current Address: The Van Andel Research Institute, 333 Bostwick, NE Grand Rapids, MI 49503, USA

For all author emails, please log on.

Genome Biology 2001, 2:research0004-research0004.13  doi:10.1186/gb-2001-2-2-research0004


A previous version of this manuscript was made available before peer review at http://genomebiology.com/2000/1/6/preprint/0001/

Published: 22 January 2001

Abstract

Background

We have developed and tested a method for printing protein microarrays and using these microarrays in a comparative fluorescence assay to measure the abundance of many specific proteins in complex solutions. A robotic device was used to print hundreds of specific antibody or antigen solutions in an array on the surface of derivatized microscope slides. Two complex protein samples, one serving as a standard for comparative quantitation, the other representing an experimental sample in which the protein quantities were to be measured, were labeled by covalent attachment of spectrally resolvable fluorescent dyes.

Results

Specific antibody-antigen interactions localized specific components of the complex mixtures to defined cognate spots in the array, where the relative intensity of the fluorescent signal representing the experimental sample and the reference standard provided a measure of each protein's abundance in the experimental sample. To test the specificity, sensitivity and accuracy of this assay, we analyzed the performance of 115 antibody/antigen pairs. 50% of the arrayed antigens and 20% of the arrayed antibodies provided specific and accurate measurements of their cognate ligands at or below concentrations of 0.34 μg/ml and 1.6 μg/ml, respectively. Some of the antibody/antigen pairs allowed detection of the cognate ligands at absolute concentrations below 1 ng/ml, and partial concentrations of 1 part in 106, sensitivities sufficient for measurement of many clinically important proteins in patient blood samples.

Conclusions

These results suggest that protein microarrays can provide a practical means to characterize patterns of variation in hundreds of thousands of different proteins in clinical or research applications.