Figure 2.

Hybridization of mouse TestisFilter against mouse testicular RNA. Eight-week-old male mice were dosed for 14 days via gavage with (a) water (control) or (b) 216 mg/kg acid BCA. Four hours after the final dose, total RNA was extracted from half a testis from each animal, and 1 μg was used to make [³³P]CTP-labeled cDNA using reverse transcription. The labeled cDNA population was hybridized against the filter overnight at 42°C. After washing, the image was captured on a Kodak phosphorimaging screen using three days of exposure and visualized using a BioRad FX phosphorimager. Selected genes with demonstrably altered expression in the BCA-treated versus control testis are highlighted (a-f).