Figure 3.

POPO-3 iodide staining of mouse TestisSlide array. POPO-3 iodide was diluted 1:10,000 in water. The TestisSlide was wetted with water and incubated in the POPO-3 solution with gentle agitation for 30 min at room temperature. Excess stain was washed away for 10 min under a gentle flow of reverse osmosis-distilled water, and the image captured using a ScanArray 4000. The number at the bottom of the TestisSlide is a unique identifier, in numeric and barcode form, for this particular slide and can be used to quickly link the image to the specific experimental conditions used to generate it.