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Genome-wide analysis of protein-DNA interactions in living cells

B Franklin Pugh and David S Gilmour*

Author Affiliations

Center for Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA

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Genome Biology 2001, 2:reviews1013-reviews1013.3 doi:10.1186/gb-2001-2-4-reviews1013

Published: 4 April 2001

Abstract

Understanding the regulation of gene expression requires an analysis of gene-specific transcription factors. This review highlights recent work that uses protein-DNA crosslinking, immunoprecipitation and DNA microarrays to determine the binding sites for specific transcription factors throughout the yeast genome.