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Mitochondrial DNA insertions

Jonathan B Weitzman

Author Affiliations

Genome Biology 2001, 2:spotlight-20010420-01  doi:10.1186/gb-spotlight-20010420-01


The electronic version of this article is the complete one and can be found online at:


Published:20 April 2001

© 2001 BioMed Central Ltd

Research news

There is evidence for substantial transfer of mitochondrial DNA (mtDNA) to the nuclear genome in plants. Analysis of the recently completed Arabidopsis thaliana genome sequence indicated a mtDNA insertion of 270 kilobases (kb), larger than previously described mitochondria-to-nuclear DNA insertions. In the April 24 Proceedings of the National Academy of Sciences, Stupar et al. present a detailed cytological characterization of the mtDNA insertion in chromosome 2 of A. thaliana (Proc Natl Acad Sci USA 2001, 98:5099-5103). Using fiber-fluorescence in situ hybridization (fiber-FISH) they show that the insertion is about 620 kb, or 2.3 times the size estimated in original measurements. The authors suggest that the complex and repetitive nature of inserted mtDNA can result in misleading estimates of insert length, and that fiber-FISH offers a high-resolution tool for genome analysis.

References

  1. The mitochondrial gene encoding ribosomal protein S12 has been translocated to the nuclear genome in Oenothera.

    PubMed Abstract OpenURL

  2. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.

    PubMed Abstract | Publisher Full Text OpenURL

  3. [http://www.pnas.org/] webcite

    Proceedings of the National Academy of Sciences