Table 2

Comparison of fold change between oligonucleotide arrays and RT-PCR

Avg Diff (array)

Fold change

Gene

hs-wt

hs-lab

Array

RT-PCR

lab

41

1078

26.3

55.7

swa

20

406

20.3

18.4

UbcD4

44

423

9.6

6.5

twe

20

132

6.6

4.9

cycB

243

1344

5.5

4.6

Uch

61

312

5.1

12.1

sqd

373

370

1.0

1.1

scrt

225

79

-2.9

-3.7

Pepck

610

171

-3.6

-4.6

RT-PCR was performed on cDNA derived from heat-shocked wild-type embryos and heat-shocked hs-lab embryos. Fold changes determined by RT-PCR are represented as the mean values of eight independent replicates, derived from two different cDNA preparations. Avg Diff, absolute average difference value (see Materials and methods).

Leemans et al. Genome Biology 2001 2:research0015.1   doi:10.1186/gb-2001-2-5-research0015