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Finger arrays

Jonathan B Weitzman

Genome Biology 2001, 2:spotlight-20010614-01  doi:10.1186/gb-spotlight-20010614-01

The electronic version of this article is the complete one and can be found online at:


Published:14 June 2001

© 2001 BioMed Central Ltd

Research news

In the June 19 issue of Proceedings of the National Academy of Sciences, Bulyk et al. describe the use of double-stranded DNA microarrays to study the sequence specificity of DNA-protein interactions (Proc Natl Acad Sci USA 2001, 98:7158-7163). They chose to look at recognition by the zinc-finger, a domain found in many human transcription factors. They examined the binding specificities of the Zif268 protein and mutants that vary in the second of three zinc fingers. Zif268 proteins bound to DNA spots were detected with by immunoflourescence. Bulyk et al. demonstrate that the relative fluorescence intensities correlated well with DNA-binding affinities. The microarray-based assay could distinguish proteins with very similar DNA-binding specificities and were useful in determining the sequence preferences of libraries of DNA-binding domains. The authors suggest that microarrays could be used to characterize a range of DNA-binding proteins and for genome-wide analysis of transcription factor binding sites.

References

  1. [http://www.pnas.org] webcite

    Proceedings of the National Academy of Sciences

  2. Quantifying DNA-protein interactions by double-stranded DNA arrays.

    PubMed Abstract | Publisher Full Text OpenURL

  3. Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.

    PubMed Abstract OpenURL