Email updates

Keep up to date with the latest news and content from Genome Biology and BioMed Central.

Research news

Fast forward to a sensitive test for prion diseases

Tudor Toma

Author affiliations

Citation and License

Genome Biology 2001, 2:spotlight-20010615-01  doi:10.1186/gb-spotlight-20010615-01


The electronic version of this article is the complete one and can be found online at:


Published:15 June 2001

© 2001 BioMed Central Ltd

Research news

Prion diseases such as scrapie, bovine spongiform encephalopathy (BSE) and variant Creutzfeldt-Jakob disease can currently be diagnosed only by post-mortem examination of brain tissue. The trace amounts of prions in the blood of infected individuals remain beyond detection. In the June 14 Nature, Gabriela Saborio and colleagues at the Serono Pharmaceutical Research Institute in Geneva, Switzerland, report that minute amounts of prion protein (PrPSc) can convert large amounts of normal protein (PrPC) into its faulty state in vitro and this could be applied to the detection of the prions in spinal fluid or blood.

The procedure that Saborio et al. have developed is conceptually analogous to polymerase chain reaction cycling. Aggregates formed when PrPSc is incubated with PrPC are disrupted by sonication to generate multiple smaller units for the continued formation of new PrPSc. After cyclic amplification, they found that 97% of the protease-resistant PrP present in the sample corresponded to newly converted protein (Nature 2001, 411:810-813).

"Accelerating the prion natural conversion process in the laboratory can compress years of [real] time into a few hours," said Claudio Soto, the senior author of the paper. The method may also provide an opportunity to determine whether PrPSc replication results in the generation of infectivity in vitro.

References

  1. [http://www.nature.com/nature] webcite

    Saborio GP, Permanne B, Soto C: Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding. Nature 2001, 411:810-813.

  2. [http://www.spri.serono.com/] webcite

    Serono Pharmaceutical Research Institute