Table 2

Fold differences in gene expression in human Th1/Th2 cells

Oligonucleotide

Real-time RT-PCR

array

Preference

Gene

Fold difference

Fold difference

SD

SD

Fold

Statistical

deltaCT (Th1)

deltaCT (Th2)

by microarray

by real-time

(average of

(average of

difference

significance§

(individual 1)

RT-PCR

individuals

individuals

(average of

(individual 1)

1,2,3)

1,2,3)

individuals 1,2,3)


Th1 >Th2

GM-CSF

4.1

3.6

6.73

0.80

8.86

0.83

4.4

**

TNF-α

12.3

6.2

2.91

0.28

4.40

0.92

2.8

**

RANTES

3.1

4.0

1.23

0.72

3.88

0.71

6.3

**

CCR1

5.1

122.0

7.18

0.85

12.73

1.95

46.7

**

CCR2

8.9

30.7

7.57

0.55

10.95

2.10

10.4

**

CCR5

1.9

109.0

10.64

0.46

15.08

1.92

21.7

**

IκB

1.7

1.4

1.76

0.65

2.92

0.55

2.2

**

TIMP-1

6.6

1.2

3.39

0.48

3.48

0.80

1.1

n.s.

Caspase-1

2.9

1.9

8.08

0.72

8.87

0.75

1.7

**

Clusterin

2.4

1.4

3.50

0.60

4.02

0.12

1.4

**

Th2 >Th1

IL-4R

7.0

2.3

7.90

0.48

6.31

1.01

3.0

n.s.

FGFR

3.8

1.9

10.10

0.53

9.35

0.18

1.7

**

CCR4

3.1

3.2

7.37

0.47

5.23

0.18

4.3

**

STAT4

1.8

1.7

7.07

0.43

6.06

0.55

2.0

**


For calculations, see Materials and methods. Same sample measured with both methods. §Data from Th1/Th2 culture samples representing three individuals were used. According to analysis of variance: n.s., not significant (p > 0.05); *significant (0.01 <p < 0.05); **highly significant (p < 0.01).

Hamalainen et al. Genome Biology 2001 2:research0022.1-research0022.11   doi:10.1186/gb-2001-2-7-research0022

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