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The presence of chorismate mutase and aromatic aminotransferase in the periplasmic fraction of Pseudomonas aeruginosa |
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| Fractions |
Enzymes |
Total enzyme activities† |
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|
|
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| PA01 |
PAT1051 |
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|
|
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| Periplasmic fraction |
DT |
Total PDT‡ |
1154 |
1154 |
| *PheC§ (marker) |
1154 |
1154 |
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| PheA¶ |
0 |
0 |
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| *AroQ |
184 |
81 |
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| AAT |
181 |
244 |
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| SDH (marker) |
13 |
38 |
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| Cytoplasmic fraction |
DT |
Total PDT‡ |
1373 |
71 |
| *PheC§ (marker) |
50 |
60 |
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| PheA¶ |
1323 |
11 |
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| AroQ• |
605 |
22 |
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| AAT |
862 |
2528 |
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| SDH (marker) |
375 |
478 |
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| Whole-cell extract |
DT |
Total PDT‡ |
2706 |
617 |
| *PheC§ |
1220 |
604 |
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| PheA¶ |
1486 |
13 |
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| *AroQ + AroQ• |
734 |
88 |
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| AAT |
905 |
3714 |
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| SDH |
402 |
512 |
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†Total enzyme activities are expressed as units (nmol of product permin) per 150 ml of culture. ‡Prephenate dehydratase activity assayed in the absence of L-phenylalanine. §Prephenate dehydratase activity assayed in the presence of 1 mM L-phenylalanine. ¶Calculated as 'Total PDT' minus '*PheC'. DT,dehydratase; PDT, prephenate dehydratase; *PheC, cyclohexadienyl dehydratase; PheA, prephenate dehydratase domain of AroQPheA; AroQ, chorismate mutase domain of aroQPheA; *AroQ, periplasmic chorismate mutase; AAT, aromatic aminotransferase; SDH, shikimate dehydrogenase. | ||||
Calhoun et al. Genome Biology 2001 2:research0030.1 doi:10.1186/gb-2001-2-8-research0030 |
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