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Brainy stem cells

Jonathan B Weitzman

Author Affiliations

Genome Biology 2001, 2:spotlight-20010816-02  doi:10.1186/gb-spotlight-20010816-02

The electronic version of this article is the complete one and can be found online at:


Published:16 August 2001

© 2001 BioMed Central Ltd

Research news

The search is on to identify and locate neural stem cells (NSCs). In the August 16 issue of Nature, Rodney Rietze and colleagues at The Walter and Eliza Hall Institute for Medical Research Australia, report the isolation of a pluripotent stem cell population from mouse brains (Nature 2001, 412:736-739). They performed a series of enrichment steps to purify NSCs from the ependymal and subventricular zones of the lateral ventricular walls. Flow cytometry was used to select a population expressing low amounts of peanut agglutinin (PNAlo) and heat-stable antigen (HSAlo). Although these cells represent just 0.27% of the unsorted population, they contain predominantly NSCs and account for 63.2% of the total NSC activity. The neural stem cells gave rise to neurospheres containing neurons, astrocytes and oligodendrocytes. Rietze et al. demonstrate that this cell population is severely reduced (6-fold) in the querkopf mutant mouse strain which exhibits stem cell deficiency. The isolated NSC could differentiate into neurons in vivo and into non-neural cell types in vitro. Thus, pluripotent stem cells (PNAlo HSAlo netrin+) can be efficiently isolated from the subventricular zone of the forebrain.

References

  1. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.

    PubMed Abstract OpenURL

  2. [http://www.nature.com] webcite

    Nature

  3. [http://www.wehi.edu.au] webcite

    The Walter and Eliza Hall Institute for Medical Research

  4. Querkopf, a MYST family histone acetyltransferase, is required for normal cerebral cortex development.

    PubMed Abstract | Publisher Full Text OpenURL