Table 1

Molecular data of polymorphisms on 3R

All the polymorphisms analyzed between the FRT 82B chromosome and the marker chromosome are shown. The table lists the approximate cytological location, the accession number of the genomic scaffold sections, the base-pair position within the section and the sequence of the polymorphisms. In 75% of the cases, the polymorphisms are real SNPs, where one nucleotide is replaced by another. In the other 25%, the nature of the polymorphisms is more complex, and involves multiple base changes or small deletions/insertions. When the polymorphism creates an RFLP (in about 50% of the cases), the experimentally tested enzyme is shown on the right. In four cases, a 2% agarose gel could resolve the migration difference of the two PCR products (when the polymorphisms were deletions of at least 20 bp). In these cases, RFLP patterns were not tested (indicated by ND^*). The last column indicates the pattern of the TM3 balancer chromosome on either RFLP or SSCP gels. M indicates that the TM3 band co-migrates with the marker chromosome and FRT, that it co-migrates with the FRT chromosome. In a few cases, the TM3 migration pattern was different from both of these chromosomes, sindicating the presence of another allele on the balancer.

Martin et al. Genome Biology 2001 2:research0036.1   doi:10.1186/gb-2001-2-9-research0036