Table 1

RNAi with C. elegans ubiquitin-conjugating enzyme genes
Cosmid
 Gene name
 dsRNA source
 Fragment size (bp)
 Chromosome
 Number of RNAi trials
 Embryonic lethality %
 Secondary phenotype
 Embryonic lethality (%) brood A
 Embryonic lethality (%)brood B
 Secondary phenotype





Feeding method
 Injection method





UBC proteins









     B0403.2
 ubc-17
 gen PCR
 591
 X
 2
 3.4
 Normal
 -
 -
 -
     C06E2.3
 ubc-21
 gen PCR
 1131
 X
 2
 0
 Normal
 -
 -
 -
     C06E2.7
 ubc-22
 gen PCR
 1075
 X
 2
 0
 Normal
 -
 -
 -
     C28G1.1
 ubc-23
 gen PCR
 491
 X
 2
 0
 Normal
 -
 -
 -
     C35B1.1
 ubc-1
 pR1.7
 593
 IV
 2
 0
 Normal
 0
 0
 Normal
     D1022.1
 ubc-6
 yk640 h7
 750
 II
 2
 6.9
 Normal
 -
 -
 -
     F29B9.6
 ubc-9
 yk312e11
 1160
 IV
 4
 60.2
 Evl, abnormal morphology
 2.7
 18.2
 Evl, Egl, abnormal morphology
     F40G9.3
 ubc-20
 yk246g3
 835
 III
 4
 0
 Rare arrest at L3 stage
 4.8
 0
 Some arrest at L3 to L4 stage*
     F49E12.4
 ubc-24
 gen PCR
 842
 II
 2
 0
 Normal
 -
 -
 -
     F58A4.10
 ubc-7
 yk486g12
 500
 III
 2
 0
 Normal
 -
 -
 -
     M7.1
 let-70
 cDNA
 444
 IV
 -
 -
 -
 -
 51
 L3 stage arrest

(ubc-2)








     R01H2.6
 ubc-18
 yk461f2
 584
 III
 2
 0
 Normal
 -
 -
 -
     R09B3.4
 ubc-12
 yk486f10
 630
 I
 6
 85.3
 Evl, abnormal morphology
 5.8
 52.3
 Evl, abnormal morphology
     Y110A2AR.2
 ubc-15
 yk602e10
 644
 II
 2
 0
 Normal
 -
 -
 -
     Y54E5B.4
 ubc-16
 yk137b4
 679
 I
 2
 0
 Normal
 1.0
 1.4
 Normal
     Y54G2A.23
 ubc-13
 yk659d2
 499
 IV
 2
 0
 Normal
 -
 -
 -
     Y69H2.6
 ubc-19
 yk290g1
 635
 V
 2
 0
 Normal
 0.8
 1.1
 Normal
     Y71G12B.15
 ubc-3
 yk255b2
 1053
 I
 2
 0
 Normal
 1.6
 2.0
 Normal
     Y87G2A.9
 ubc-14
 yk573c8
 532
 I
 6
 54.1
 Abnormal morphology
 -
 -
 -
     Y94H6A.6
 ubc-8
 gen PCR
 890
 IV
 2
 0
 Normal
 -
 -
 -
UEV proteins









     F39B2.2
 uev-1
 yk593d12
 499
 I
 2
 0
 Normal
 -
 -
 -
     F56D2.4
 uev-2
 gen PCR
 1074
 III
 2
 0
 Normal
 -
 -
 -
     F26H9.7
 uev-3
 RT-PCR
 660
 I
 2
 0
 Normal
-
 --

Gene names have been assigned as indicated in the text. DNA fragments of the indicated sizes were obtained from the cDNA sources shown or from fragments obtained by genomic PCR (gen PCR), or by reverse transcription from RNA (RT-PCR). Phenotypes include Evl (everted vulva) and Egl (egg-laying defect). Embryonic lethality for broods A and B are shown separately. A dash indicates experiment not done. *Variable penetrance. Two potential C. elegansubc genes that have recently come to light are not included in this table. F25H2.8 encodes a protein that is 25% identical to UBC-1 over 100 amino acids spanning the active site. This UBC domain is not recognized by Pfam, but is recognized by SMART (UBCc domain). The protein is 56% identical in amino-acid sequence to Drosophila BG:25E8.2 and therefore belongs in group I of Figures 1 and 2. RNAi experiments with F25H2.8 produce paralysis in the adult stage and premature death of the hermaphrodites 4 to 5 days after the beginning of oviposition [85]. YAC clone Y110A2AM may contain another ubc gene (currently uncurated) encoding a protein 52% identical over 143 amino acids to Y110A2AR.2 (group XVIII).

Jones et al. Genome Biology 2001 3:research0002.1   doi:10.1186/gb-2001-3-1-research0002

Open Data