Table 2

RNAi with C. elegans ubiquitin-activating enzyme genes and ubiquitin-like genes

Cosmid

Gene name

dsRNA source

Chromosome

Number of RNAi trials

Embryonic lethality (%)

Secondary phenotype

Ubiquitin-activating enzymes

F11H8.1

uba-3

gen PCR

III

2

41

Evl

C26E6.8

ula-1

yk433e12

III

2

6

Evl

W02A11.4

uba-2

yk632h1

V

2

40

Evl

C47E12.4

uba-1

yk9g12

IV

2

100

Low brood size, death

C08B6.9

aos-1

-

V

Not done

-

-

Ubiquitin-like fusion proteins

B0303.4

yk490f10

III

2

0

None

C16C8.4

yk262b4

II

2

0

None

F49C12.9

yk162f5

IV

2

0

None

ZK688.5

yk16d10

III

2

2.5

None

Small ubiquitin-like proteins

F45H11.2

ned-8

T01966

I

1

2

Evl, abnormal morphology

K12C11.2

sumo

yk222g1

I

2

100

Evl, abnormal morphology

F46F11.4

gen PCR

I

2

0

None

F32H5.3

yk534f3

V

2

0

None

DNA fragments used to generate the dsRNA were obtained from the indicated sources, some fragments were generated by genomic PCR (gen PCR). Phenotypes include Evl (everted vulva). A dash indicates experiment not done.

Jones et al. Genome Biology 2001 3:research0002.1   doi:10.1186/gb-2001-3-1-research0002