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Networks in the yeast genome

Jonathan B Weitzman

Author Affiliations

Genome Biology 2002, 3:spotlight-20021029-01  doi:10.1186/gb-spotlight-20021029-01


The electronic version of this article is the complete one and can be found online at:


Published:29 October 2002

© 2002 BioMed Central Ltd

Research news

We are far from understanding the how complex transcriptional programs are generated from genomic information. In the October 25 Science, Lee et al. report how they constructed a map of transcriptional regulatory networks in Saccharomyces cerevisiae by identifying all the sites bound by a arge number of transcriptional regulators (Science 2002, 298:799-804). They performed genome-wide location analysis to find binding sites for all 141 transcription factors listed in the Yeast Proteome Database. They then introduced a myc epitope tag into the genomic sequences encoding each factor. Using immunoprecipitation and microarray analysis, almost 4,000 regulator-DNA interactions could be defined. More than a third of all yeast genes were bound by one or more of the regulators and many were bound by multiple factors. The average number of gene promoters bound by an individual factor was 38. These data were used to construct regulatory network that are made up of different motifs - autoregulation, multicomponent loops, feedforward loops, and so on. Lee et al. studied cell-cycle-regulated genes to illustrate how an automated approach combining genome-wide location and expression data can be used to generate complex regulatory network models.

References

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