Table 3

List of previously unknown genes that can be annotated using GPPP

E. coli gene name (Genbank id)
Previous annotation
Predicted function by GPPP
Additional evidences from BLAST/Pfam/COG

b1681 (1787971)
Hypothetical protein
Transport system, membrane protein
COG0719: predicted membrane components of ABC-transporter SufB
b1683 (1787973)
Hypothetical protein
Transport system, membrane protein

b2608 (1788960)
Hypothetical protein
RNA processing
Pfam domain: RimM. RimM is essential for processing of 16S rRNA.
b2766 (1789125)
Hypothetical protein
Flavorprotein, electron transport
COG0644:dehydrogenase/flavoprotein
b0407 (1786608)
Hypothetical protein
Protein secretion
Pfam domain: DUF219, (uncharacterized secreted protein)
b1395 (1787661)
Putative enzyme
Part of fad operon
BLAST: 3-hydroxyacyl-CoA dehydrogenase
b2341 (1788682)
Putative enzyme
Part of fad operon
BLAST: 3-hydroxyacyl-CoA dehydrogenase; enoyl-CoA isomerase/hydrotase
b0284 (1786478)
Hypothetical protein
Neighboring with xanthine dehydrogenase gene (b0286)
BLAST: Putative oxidoreductase
b2866 (1789230)
Hypothetical protein
Neighboring with xanthine dehydrogenase (b2868)
BLAST: Probable aldehyde oxidase and xanthine dehydrogenase family protein
b1674 (1787963)
Hypothetical protein
Oxidoreductase
BLAST: Aldehyde ferredoxin oxidoreductase
b2371 (1788714)
Hypothetical protein
Enzyme in carnitine metabolism
Pfam domain: CAIB-BAIF, domain involved in carnite metabolism
b2374 (1788717)
Hypothetical protein
Enzyme in carnitine metabolism
Pfam domain: CAIB-BAIF, domain involved in carnite metabolism
b2125 (2367130)
Hypothetical protein
Response regulator in 2-component system
Pfam domains: response-reg, REC probable cheY receiver domain
b0936 (1787167)
Hypothetical protein
Transport system
Pfam domain: PBPb, transport system related
b2075 (1788390)
Hypothetical protein
Integral transmembrane protein
Pfam: ACR_tran, SecD_SecF, Patched, transmembrane domain related
b2076 (1788391)
Hypothetical protein
Integral transmembrane protein
Pfam: ACR_tran
b1086 (1787327)
Hypothetical protein
RNA processing
BLAST: RNA pseudouridylate synthase

Zheng et al. Genome Biology 2002 3:research0060.1   doi:10.1186/gb-2002-3-11-research0060