Table 2

QTL genes with nonsynonymous changes
(a) Altered genes within the chronic alcohol withdrawal QTL on proximal chromosome 1

Gene
 In silico percent coverage*
 Amino-acid variations


Symbol
 Gene ID
 Location
 B6
 D2
 Both

mCG55019
 30,510,903 - 30,520,321
 100
 100
 100
 M72V†

mCG52566
 31,637,483 - 31,666,730
 95
 95
 95
 S6A

mCG49360
 32,140,873 - 32,141,648
 100
 87
 87
 P74L†, C123R†

mCG66997
 36,796,676 - 36,805,521
 100
 100
 100
 stop61Y

mCG4911
 39,571,491 - 39,572,134
 54
 100
 54
 R74H, E79D
Slc5a7
 NM_022025
 Uncertain
 100
 99 (100)
 99 (100)
 R38H†
(b) Altered genes within the sex-limited chronic alcohol withdrawal QTL on chromosome 13

Gene
 In silico percent coverage*
 Amino-acid variations


Symbol
 Gene ID
 Location
 B6
 D2
 Both

mCG22018
 54,248,540 - 54,266,542
 100
 86
 86
 I155F
Ptch
 mCG21490
 54,702,052 - 54,733,739
 79
 68
 68
 T1267N

mCG67381
 54,910,868 - 54,921,322
 95
 95
 95
 N48D

mCG49128
 55,141,914 - 55,156 036
 82
 100
 82
 S38G, H96R
Hsd17b3
 NM_008291
 55,258,374 - 55,287,747
 69 (100)
 61 (100)
 45 (100)
 C95R†

mCG67239
 55,349,545 - 55,350,013
 100
 100
 100
 T47M, P124R‡
Habp4
 NM_019986
 55,358,811 - 55,383,494
 89 (96)
 90 (96)
 84 (96)
 A65-(in/del)†, P187L†, A266T†

mCG65010
 55,397,330 - 55,399,305
 84
 100
 84
 S48N†, R76W†

mCG51118
 55,652,165 - 55,652,438
 31
 100
 31
 F6L‡

mCG50935
 55,841,328 - 55,842,516
 100
 100
 100
 A210V

mCG50411
 56,005,687 - 56,054,181
 100
 66
 66
 G200E†

mCG55714
 56,067,926 - 56,074,495
 96
 90
 90
 S99P‡, L361S‡, Q557R‡, A851V‡

mCG59053
 56,106,754 - 56,107,033
 84
 100
 84
 S64G‡

mCG59052
 56,122,823 - 56,169,063
 93
 86
 79
 W53R, M62V, T17I, V197A, T198P, A324S, D645N, R728G, W846R†
Nrif-2
 AJ319726
 56,376,102 - 56,425,620
 96 (100)
 78 (100)
 74 (100)
 A491T†, Q593R†, I679V†
Ptdss1
 mCG67987
 57,190,261 - 57,251,587
 100
 89
 89
 G322C†

mCG67995
 57,426,346 - 57,427,146
 100
 83
 83
 G4E

mCG67997
 57,732,668 - 57,740,675
 53 (100)
 100
 53 (100)
 K24T†, L30R†, S36P†, I48S†, K62R†, V89E†, N94T†
Srd5a1
 mCG7698
 58,982,812 - 59,009,801
 78 (100)
 78 (100)
 78 (100)
 R7C†, V142I†, E176D†

Gene IDs beginning with the letters mCG are Celera gene ID numbers; all other gene ID numbers are GenBank accession numbers for transcript sequences. Although genes are identified by the Celera genomic (mCG) ID, transcript sequence was used as query for the BLAST searches for each gene. All data were retrieved from Celera and NCBI between August 2001 and April 2002. Only changes where the reading frame is known and sequence is available in both B6 and D2 are displayed. The B6 amino acid is given first, then the position, followed by the D2 amino acid. *Numbers in parentheses indicate percent coverage after in-house sequencing. †These changes were verified by in-house sequencing. ‡These changes are supported by only a single read in either B6 or D2 and have not been verified by in-house sequencing.

Marshall et al. Genome Biology 2002 3:research0078.1   doi:10.1186/gb-2002-3-12-research0078