Figure 4.

Comparison of image and microarray data for gene CG4702. (a) A microarray expression profile of the gene CG4702, represented by EST clone LD43816. Consecutive time points representing RNA samples from differentially aged 1-h embryo collections are presented on the x-axis, while the y-axis represents the scale of absolute expression indexes as analyzed by dChip analysis software [37]. Error bars represent standard error of the measurements, which were carried out independently on three separate embryo collections; the number next to each bar is the mean of the three measurements. The color of the bar reflects the categorization of each measurement on the basis of a statistical analysis performed by the Affymetrix Microarray Suite software package. Green represents present; red, absent. Because of the sensitivity limitations of the GeneChip measurements and the conservative nature of the software, absent calls do not necessarily imply lack of gene expression. The secondary x-axis correlates the microarray sample time points with stages of embryogenesis. (b) Images of six staged embryos illustrating expression during development of the same gene characterized above by microarray analysis. Each image comes from a different stage range (stages 1-3, 4-6, 7-8, 9-10, 11-12, and 14-16). Lateral view, anterior to the left.