Genome Biology

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Finishing a whole-genome shotgun: Release 3 of the Drosophila melanogaster euchromatic genome sequence

Susan E Celniker*, David A Wheeler, Brent Kronmiller, Joseph W Carlson, Aaron Halpern, Sandeep Patel, Mark Adams, Mark Champe, Shannon P Dugan, Erwin Frise, Ann Hodgson, Reed A George, Roger A Hoskins, Todd Laverty, Donna M Muzny, Catherine R Nelson, Joanne M Pacleb, Soo Park, Barret D Pfeiffer, Stephen Richards, Erica J Sodergren, Robert Svirskas, Paul E Tabor, Kenneth Wan, Mark Stapleton, Granger G Sutton, Craig Venter, George Weinstock, Steven E Scherer, Eugene W Myers, Richard A Gibbs and Gerald M Rubin

Genome Biology 2002, 3:research0079-0079.14 doi:10.1186/gb-2002-3-12-research0079

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Hundreds of putatively functional small open reading frames in Drosophila

Emmanuel Ladoukakis, Vini Pereira, Emile G Magny, Adam Eyre-Walker, Juan Pablo Couso Genome Biology 2011, 12:R118 (25 November 2011)

The Drosophila genome contains hundreds of small open reading frames with putative functionality

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Motif composition, conservation and condition-specificity of single and alternative transcription start sites in the Drosophila genome

Elizabeth A Rach, Hsiang-Yu Yuan, William H Majoros, Pavel Tomancak, Uwe Ohler Genome Biology 2009, 10:R73 (9 July 2009)

A map of transcription start sites across the Drosophila genome, providing insights into initiation patterns and spatiotemporal conditions.

Research article   Open Access

Chromosome level assembly of the hybrid Trypanosoma cruzi genome

D Brent Weatherly, Courtney Boehlke, Rick L Tarleton BMC Genomics 2009, 10:255 (1 June 2009)

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A whole-genome assembly of the domestic cow, Bos taurus

Aleksey V Zimin, Arthur L Delcher, Liliana Florea, David R Kelley, Michael C Schatz, Daniela Puiu, Finnian Hanrahan, Geo Pertea, Curtis P Van Tassell, Tad S Sonstegard, Guillaume Marçais, Michael Roberts, Poorani Subramanian, James A Yorke, Steven L Salzberg Genome Biology 2009, 10:R42 (24 April 2009)

A cow whole-genome assembly of 2.86 billion base pairs that closes gaps and corrects previously-described inversions and deletions as well as describing a portion of the Y chromosome.

Methodology article   Open Access Highly Accessed

Quantitative measures for the management and comparison of annotated genomes

Karen Eilbeck, Barry Moore, Carson Holt, Mark Yandell BMC Bioinformatics 2009, 10:67 (23 February 2009)

Database   Open Access

The Drosophila melanogaster PeptideAtlas facilitates the use of peptide data for improved fly proteomics and genome annotation

Sandra N Loevenich, Erich Brunner, Nichole L King, Eric W Deutsch, Stephen E Stein, Ruedi Aebersold, Ernst Hafen BMC Bioinformatics 2009, 10:59 (11 February 2009)

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Ancora: a web resource for exploring highly conserved noncoding elements and their association with developmental regulatory genes

Pär G Engström, David Fredman, Boris Lenhard Genome Biology 2008, 9:R34 (15 February 2008)

Ancora is a web resource that provides data and tools for exploring genomic organization of highly conserved noncoding elements for multiple genomes.

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Identification of novel regulatory factor X (RFX) target genes by comparative genomics in Drosophila species

Anne Laurençon, Raphaëlle Dubruille, Evgeni Efimenko, Guillaume Grenier, Ryan Bissett, Elisabeth Cortier, Vivien Rolland, Peter Swoboda, Bénédicte Durand Genome Biology 2007, 8:R195 (17 September 2007)

An RFX-binding site is shown to be conserved in the promoters of a subset of ciliary genes and a subsequent screen for this site in two Drosophila species identified novel RFX target genes that are involved in sensory ciliogenesis.

Research article   Open Access

Complex regulation and multiple developmental functions of misfire, the Drosophila melanogaster ferlin gene

Michelle K Smith, Barbara T Wakimoto BMC Developmental Biology 2007, 7:21 (26 March 2007)

Method   Open Access

A haplome alignment and reference sequence of the highly polymorphic Ciona savignyi genome

Kerrin S Small, Michael Brudno, Matthew M Hill, Arend Sidow Genome Biology 2007, 8:R41 (20 March 2007)

The high degree of polymorphism in the genome of the sea squirt Ciona savignyi complicated the assembly of sequence contigs, but a new alignment method results in a much improved sequence.

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Fast sequence evolution of Hox and Hox-derived genes in the genus Drosophila

Sònia Casillas, Bárbara Negre, Antonio Barbadilla, Alfredo Ruiz BMC Evolutionary Biology 2006, 6:106 (12 December 2006)

Hox genes in fruit flies are evolving rapidly despite their complex expression patterns and conserved role in development, an unexpected phenomenon that is only partly explained by the presence of microsatellites within the coding sequences.

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Recurrent insertion and duplication generate networks of transposable element sequences in the Drosophila melanogaster genome

Casey M Bergman, Hadi Quesneville, Dominique Anxolabéhère, Michael Ashburner Genome Biology 2006, 7:R112 (29 November 2006)

An analysis of high-resolution transposable element annotations in Drosophila melanogaster suggests the existence of a global surveillance system against the majority of transposable elements families in the fly.

Research   Open Access

Fine scale structural variants distinguish the genomes of Drosophila melanogaster and D. pseudoobscura

Stuart J Macdonald, Anthony D Long Genome Biology 2006, 7:R67 (27 July 2006)

Comparative genomics reveals fine-scale structural variants, including microinversions, distinguishing two diverged Drosophila species

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The cnidarian-bilaterian ancestor possessed at least 56 homeoboxes: evidence from the starlet sea anemone, Nematostella vectensis

Joseph F Ryan, Patrick M Burton, Maureen E Mazza, Grace K Kwong, James C Mullikin, John R Finnerty Genome Biology 2006, 7:R64 (24 July 2006)

The first near-complete set of homeodomains from a non-bilaterian animal is described.

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Global analysis of X-chromosome dosage compensation

Vaijayanti Gupta, Michael Parisi, David Sturgill, Rachel Nuttall, Michael Doctolero, Olga K Dudko, James D Malley, P Scott Eastman, Brian Oliver Journal of Biology 2006, 5:3 (16 February 2006)

In Drosophila germ cells, X chromosome genes are upregulated to balance their expression with that of autosomes; the same happens in somatic cells of mice and nematodes.

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Challenging the spliceosome machine

Michael Weir, Matthew Eaton, Michael Rice Genome Biology 2006, 7:R3 (17 January 2006)

Analysis of a set of almost 25,000 donor and acceptor splice sites in Drosophila shows that information content increases near splice sites flanking very long of very short introns and exons.

Research article   Open Access

Paucity of chimeric gene-transposable element transcripts in the Drosophila melanogaster genome

Mikhail Lipatov, Kapa Lenkov, Dmitri A Petrov, Casey M Bergman BMC Biology 2005, 3:24 (12 November 2005)

Less than 1% of Drosophila transcripts contain 'chimeric' transposable elements, whereas a large proportion of mammalian genomes are known to be derived from TEs; population genetics implies that most fly gene-TE chimeras are deleterious, but a few may contribute to novel gene sequences.

Research article   Open Access

Sequence signature analysis of chromosome identity in three Drosophila species

Per Stenberg, Fredrik Pettersson, Anja O Saura, Anders Berglund, Jan Larsson BMC Bioinformatics 2005, 6:158 (23 June 2005)

Method   Open Access

Genomic analysis of heat-shock factor targets in Drosophila

Ian Birch-Machin, Shan Gao, David Huen, Richard McGirr, Robert AH White, Steven Russell Genome Biology 2005, 6:R63 (10 June 2005)

A ChIP-assay approach is used for the whole-genome transcription factor target mapping in vivo using an intact whole organism. Using this ChIP-array 141 genes are identified in a genomic search for Hsf targets in Drosophila.

Research article   Open Access

Conserved genomic organisation of Group B Sox genes in insects.

Carol McKimmie, Gertrud Woerfel, Steven Russell BMC Genetics 2005, 6:26 (19 May 2005)

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Comparison of the oxidative phosphorylation (OXPHOS) nuclear genes in the genomes of Drosophila melanogaster, Drosophila pseudoobscura and Anopheles gambiae

Gaetano Tripoli, Domenica D'Elia, Paolo Barsanti, Corrado Caggese Genome Biology 2005, 6:R11 (31 January 2005)

An analysis of nuclear-encoded oxidative phosphorylation genes in Drosophila and Anopheles reveals that pairs of duplicated genes have strikingly different expression patterns.

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A Drosophila protein-interaction map centered on cell-cycle regulators

Clement A Stanyon, Guozhen Liu, Bernardo A Mangiola, Nishi Patel, Loic Giot, Bing Kuang, Huamei Zhang, Jinhui Zhong, Russell L Finley Genome Biology 2004, 5:R96 (26 November 2004)

A Drosophila protein-protein interaction map was constructed using the LexA system, complementing a previous map using the GAL4 system and adding many new interactions.

Research article   Open Access

Genomic characterization of a repetitive motif strongly associated with developmental genes in Drosophila

Javier Costas, Cristina P Vieira, Fernando Casares, Jorge Vieira BMC Genomics 2003, 4:52 (16 December 2003)

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Computational identification of Drosophila microRNA genes

Eric C Lai, Pavel Tomancak, Robert W Williams, Gerald M Rubin Genome Biology 2003, 4:R42 (30 June 2003)

An informatic procedure has been used to analyze the euchromatic sequences of Drosophila melanogaster and D. pseudoobscura for conserved sequences that adopt an extended stem-loop structure and display other characteristics of known miRNAs.

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Impressive expressions: developing a systematic database of gene-expression patterns in Drosophila embryogenesis

Haiqiong Montalta-He, Heinrich Reichert Genome Biology 2003, 4:205 (28 January 2003)

The establishment of a database of gene-expression patterns derived from systematic highthroughput in situ hybridization studies on whole-mount Drosophila embryos vastly increases the breadth and depth that can be reached by developmental genetics.

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Heterochromatic sequences in a Drosophila whole-genome shotgun assembly

Roger A Hoskins, Christopher D Smith, Joseph W Carlson, A Bernardo Carvalho, Aaron Halpern, Joshua S Kaminker, Cameron Kennedy, Chris J Mungall, Beth A Sullivan, Granger G Sutton, Jiro C Yasuhara, Barbara T Wakimoto, Eugene W Myers, Susan E Celniker, Gerald M Rubin, Gary H Karpen Genome Biology 2002, 3:research0085-0085.16 (31 December 2002)

This article is part of a collection on Release 3 of the...

Annotation of an improved whole-genome shotgun assembly of the Drosophila melanogaster genome predicted 297 protein-coding genes and six non-protein-coding genes, including known heterochromatic genes, and regions of similarity to known transposable elements. Fluorescence in situ hybridization was used to correlate the genomic sequence with the cytogenetic map; the annotated euchromatic sequence extends into the centric heterochromatin on each chromosome arm.

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Annotation of the Drosophila melanogaster euchromatic genome: a systematic review

Sima Misra, Madeline A Crosby, Christopher J Mungall, Beverley B Matthews, Kathryn S Campbell, Pavel Hradecky, Yanmei Huang, Joshua S Kaminker, Gillian H Millburn, Simon E Prochnik, Christopher D Smith, Jonathan L Tupy, Eleanor J Whitfield, Leyla Bayraktaroglu, Benjamin P Berman, Brian R Bettencourt, Susan E Celniker, Aubrey DNJ de Grey, Rachel A Drysdale, Nomi L Harris, John Richter, Susan Russo, Andrew J Schroeder, ShengQiang Shu, Mark Stapleton, Chihiro Yamada, Michael Ashburner, William M Gelbart, Gerald M Rubin, Suzanna E Lewis Genome Biology 2002, 3:research0083-0083.22 (31 December 2002)

This article is part of a collection on Release 3 of the...

The recent completion of the Drosophila melanogaster genomic sequence to high quality, and the availability of a greatly expanded set of Drosophila cDNA sequences, afforded FlyBase the opportunity to significantly improve genomic annotations.

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Assessing the impact of comparative genomic sequence data on the functional annotation of the Drosophila genome

Casey M Bergman, Barret D Pfeiffer, Diego E Rincón-Limas, Roger A Hoskins, Andreas Gnirke, Chris J Mungall, Adrienne M Wang, Brent Kronmiller, Joanne Pacleb, Soo Park, Mark Stapleton, Kenneth Wan, Reed A George, Pieter J de Jong, Juan Botas, Gerald M Rubin, Susan E Celniker Genome Biology 2002, 3:research0086-0086.20 (30 December 2002)

This article is part of a collection on Release 3 of the...

Analysis of conservation in eight genomic regions (apterous, even-skipped, fushi tarazu, twist, and Rhodopsins 1, 2, 3 and 4) from four Drosophila species (D. erecta, D. pseudoobscura, D. willistoni, and D. littoralis) covering more than 500 kb of the D. melanogaster genome. All D. melanogaster genes (and 78-82% of coding exons) identified in divergent species such as D. pseudoobscura show evidence of functional constraint. Addition of a third species can reveal functional constraint in otherwise non-significant pairwise exon comparisons.

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The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective

Joshua S Kaminker, Casey M Bergman, Brent Kronmiller, Joseph Carlson, Robert Svirskas, Sandeep Patel, Erwin Frise, David A Wheeler, Suzanna E Lewis, Gerald M Rubin, Michael Ashburner, Susan E Celniker Genome Biology 2002, 3:research0084-0084.20 (23 December 2002)

This article is part of a collection on Release 3 of the...

Using Release 3 of the euchromatic genomic sequence of Drosophila melanogaster, 85 known and eight novel families of transposable element have been identified, varying in copy number from one to 146. A total of 1,572 full and partial transposable elements were identified, comprising 3.86% of the sequence.

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An integrated computational pipeline and database to support whole-genome sequence annotation

CJ Mungall, S Misra, BP Berman, J Carlson, E Frise, N Harris, B Marshall, S Shu, JS Kaminker, SE Prochnik, CD Smith, E Smith, JL Tupy, C Wiel, GM Rubin, SE Lewis Genome Biology 2002, 3:research0081-0081.11 (23 December 2002)

This article is part of a collection on Release 3 of the...

In the course of annotating the Drosophila melanogaster genome sequence, several new open-source software tools and a database schema to support large-scale genome annotation were developed and integrated in to a reusable software system for whole-genome annotation.

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A Drosophila full-length cDNA resource

Mark Stapleton, Joe Carlson, Peter Brokstein, Charles Yu, Mark Champe, Reed George, Hannibal Guarin, Brent Kronmiller, Joanne Pacleb, Soo Park, Ken Wan, Gerald M Rubin, Susan E Celniker Genome Biology 2002, 3:research0080-0080.8 (23 December 2002)

This article is part of a collection on Release 3 of the...

High-quality full-insert sequence for 8,921 putative full-length cDNA clones in the Drosophila Gene Collection has been generated and compared to the annotated Release 3 genomic sequence. More than 5,300 cDNAs have been identifieed that contain a complete and accurate protein-coding sequence, corresponding to at least one splice form for 40% of the predicted D. melanogaster genes.