Genome Biology

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A Drosophila full-length cDNA resource

Mark Stapleton*, Joe Carlson, Peter Brokstein, Charles Yu, Mark Champe, Reed George, Hannibal Guarin, Brent Kronmiller, Joanne Pacleb, Soo Park, Ken Wan, Gerald M Rubin and Susan E Celniker

Genome Biology 2002, 3:research0080-0080.8 doi:10.1186/gb-2002-3-12-research0080


This article is part of a series of refereed research articles from Berkeley Drosophila Genome Project, FlyBase and colleagues, describing Release 3 of the Drosophila genome, which are freely available at http://genomebiology.com/drosophila/.

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Research   Open Access Highly Accessed

Motif composition, conservation and condition-specificity of single and alternative transcription start sites in the Drosophila genome

Elizabeth A Rach, Hsiang-Yu Yuan, William H Majoros, Pavel Tomancak, Uwe Ohler Genome Biology 2009, 10:R73 (9 July 2009)

A map of transcription start sites across the Drosophila genome, providing insights into initiation patterns and spatiotemporal conditions.

Database   Open Access

The Drosophila melanogaster PeptideAtlas facilitates the use of peptide data for improved fly proteomics and genome annotation

Sandra N Loevenich, Erich Brunner, Nichole L King, Eric W Deutsch, Stephen E Stein, Ruedi Aebersold, Ernst Hafen BMC Bioinformatics 2009, 10:59 (11 February 2009)

Research article   Open Access

Complex organizational structure of the genome revealed by genome-wide analysis of single and alternative promoters in Drosophila melanogaster

Qianqian Zhu, Marc S Halfon BMC Genomics 2009, 10:9 (7 January 2009)

Research article   Open Access

Large-scale collection and annotation of full-length enriched cDNAs from a model halophyte, Thellungiella halophila

Teruaki Taji, Tetsuya Sakurai, Keiichi Mochida, Atsushi Ishiwata, Atsushi Kurotani, Yasushi Totoki, Atsushi Toyoda, Yoshiyuki Sakaki, Motoaki Seki, Hirokazu Ono, Yoichi Sakata, Shigeo Tanaka, Kazuo Shinozaki BMC Plant Biology 2008, 8:115 (12 November 2008)

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An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library

Chaofu Lu, James G Wallis, John Browse BMC Plant Biology 2007, 7:42 (31 July 2007)

In the developing endosperm of castor seeds, transcript annotation has led to the discovery that transcriptional regulation of oleate desaturase (FAD2) and oleate hydroxylase (FAH12) contribute to the accumulation of ricinoleate, an important industrial raw material.

Research   Open Access Highly Accessed

Global analysis of patterns of gene expression during Drosophila embryogenesis

Pavel Tomancak, Benjamin P Berman, Amy Beaton, Richard Weiszmann, Elaine Kwan, Volker Hartenstein, Susan E Celniker, Gerald M Rubin Genome Biology 2007, 8:R145 (23 July 2007)

Embryonic expression patterns for 6,003 (44%) of the 13,659 protein-coding genes identified in the Drosophila melanogaster genome were documented, of which 40% show tissue-restricted expression.

Method   Open Access

Correcting for sequence biases in present/absent calls

Eugene F Schuster, Eric Blanc, Linda Partridge, Janet M Thornton Genome Biology 2007, 8:R125 (26 June 2007)

Correction of non-specific binding for both PM and MM probes using probe-sequence models can partially remove the probe-sequence bias in Affymetrix microarray experiments and result in better performance of the MAS 5.0 algorithm.

Method   Open Access

An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta

John Wang, Stephanie Jemielity, Paolo Uva, Yannick Wurm, Johannes Gräff, Laurent Keller Genome Biology 2007, 8:R9 (15 January 2007)

An annotated EST resource for the fire ant Solenopsis invicta containing 21,715 ESTs, which represent 11,864 putatively different transcripts, and a corresponding cDNA microarray are described.

Method   Open Access

Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts

Florian Hahne, Dorit Arlt, Mamatha Sauermann, Meher Majety, Annemarie Poustka, Stefan Wiemann, Wolfgang Huber Genome Biology 2006, 7:R77 (17 August 2006)

A software tool for the analysis of high-throughput cell-based assays is presented.

Research article   Open Access

Generation and analysis of large-scale expressed sequence tags (ESTs) from a full-length enriched cDNA library of porcine backfat tissue

Tae-Hun Kim, Nam-Soon Kim, Dajeong Lim, Kyung-Tai Lee, Jung-Hwa Oh, Hye-Sook Park, Gil-Won Jang, Hyung-Yong Kim, Mina Jeon, Bong-Hwan Choi, Hae-Young Lee, HY Chung, Heebal Kim BMC Genomics 2006, 7:36 (27 February 2006)

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Challenging the spliceosome machine

Michael Weir, Matthew Eaton, Michael Rice Genome Biology 2006, 7:R3 (17 January 2006)

Analysis of a set of almost 25,000 donor and acceptor splice sites in Drosophila shows that information content increases near splice sites flanking very long of very short introns and exons.

Research article   Open Access

Paucity of chimeric gene-transposable element transcripts in the Drosophila melanogaster genome

Mikhail Lipatov, Kapa Lenkov, Dmitri A Petrov, Casey M Bergman BMC Biology 2005, 3:24 (12 November 2005)

Less than 1% of Drosophila transcripts contain 'chimeric' transposable elements, whereas a large proportion of mammalian genomes are known to be derived from TEs; population genetics implies that most fly gene-TE chimeras are deleterious, but a few may contribute to novel gene sequences.

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A survey of ovary-, testis-, and soma-biased gene expression in Drosophila melanogaster adults

Michael Parisi, Rachel Nuttall, Pamela Edwards, James Minor, Daniel Naiman, Jining Lü, Michael Doctolero, Marina Vainer, Cathy Chan, James Malley, Scott Eastman, Brian Oliver Genome Biology 2004, 5:R40 (1 June 2004)

A global analysis of sex-biased transcription in Drosophila shows extensive differential expression between the sexes. Most sex-differential expression is due to germ cells and nearly all genes with germline expression show sex-bias.

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parkin counteracts symptoms in a Drosophila model of Parkinson's disease

Annika FM Haywood, Brian E Staveley BMC Neuroscience 2004, 5:14 (16 April 2004)

Co-expression of the parkin gene with the alpha synuclein gene suppresses symptoms in the alpha-synuclein-dependent Drosophila model of Parkinson's disease, suggesting that up-regulation of parkin may be a means to suppress alpha-synucleinopathic Parkinson's disease in humans.

Research   Open Access

Genome-wide analysis of mRNA lengths in Saccharomyces cerevisiae

Evan H Hurowitz, Patrick O Brown Genome Biology 2003, 5:R2 (22 December 2003)

A novel 'Virtual Northern' method provides a practical and efficient method for genome-scale analysis of mRNA transcript lengths. A study in Saccharomyces cerevisiae has revealed that approximately 12-15% of the yeast genome is represented in untranslated sequences of mRNAs.

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Impressive expressions: developing a systematic database of gene-expression patterns in Drosophila embryogenesis

Haiqiong Montalta-He, Heinrich Reichert Genome Biology 2003, 4:205 (28 January 2003)

The establishment of a database of gene-expression patterns derived from systematic highthroughput in situ hybridization studies on whole-mount Drosophila embryos vastly increases the breadth and depth that can be reached by developmental genetics.

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Heterochromatic sequences in a Drosophila whole-genome shotgun assembly

Roger A Hoskins, Christopher D Smith, Joseph W Carlson, A Bernardo Carvalho, Aaron Halpern, Joshua S Kaminker, Cameron Kennedy, Chris J Mungall, Beth A Sullivan, Granger G Sutton, Jiro C Yasuhara, Barbara T Wakimoto, Eugene W Myers, Susan E Celniker, Gerald M Rubin, Gary H Karpen Genome Biology 2002, 3:research0085-0085.16 (31 December 2002)

This article is part of a collection on Release 3 of the...

Annotation of an improved whole-genome shotgun assembly of the Drosophila melanogaster genome predicted 297 protein-coding genes and six non-protein-coding genes, including known heterochromatic genes, and regions of similarity to known transposable elements. Fluorescence in situ hybridization was used to correlate the genomic sequence with the cytogenetic map; the annotated euchromatic sequence extends into the centric heterochromatin on each chromosome arm.

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Annotation of the Drosophila melanogaster euchromatic genome: a systematic review

Sima Misra, Madeline A Crosby, Christopher J Mungall, Beverley B Matthews, Kathryn S Campbell, Pavel Hradecky, Yanmei Huang, Joshua S Kaminker, Gillian H Millburn, Simon E Prochnik, Christopher D Smith, Jonathan L Tupy, Eleanor J Whitfield, Leyla Bayraktaroglu, Benjamin P Berman, Brian R Bettencourt, Susan E Celniker, Aubrey DNJ de Grey, Rachel A Drysdale, Nomi L Harris, John Richter, Susan Russo, Andrew J Schroeder, ShengQiang Shu, Mark Stapleton, Chihiro Yamada, Michael Ashburner, William M Gelbart, Gerald M Rubin, Suzanna E Lewis Genome Biology 2002, 3:research0083-0083.22 (31 December 2002)

This article is part of a collection on Release 3 of the...

The recent completion of the Drosophila melanogaster genomic sequence to high quality, and the availability of a greatly expanded set of Drosophila cDNA sequences, afforded FlyBase the opportunity to significantly improve genomic annotations.

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Assessing the impact of comparative genomic sequence data on the functional annotation of the Drosophila genome

Casey M Bergman, Barret D Pfeiffer, Diego E Rincón-Limas, Roger A Hoskins, Andreas Gnirke, Chris J Mungall, Adrienne M Wang, Brent Kronmiller, Joanne Pacleb, Soo Park, Mark Stapleton, Kenneth Wan, Reed A George, Pieter J de Jong, Juan Botas, Gerald M Rubin, Susan E Celniker Genome Biology 2002, 3:research0086-0086.20 (30 December 2002)

This article is part of a collection on Release 3 of the...

Analysis of conservation in eight genomic regions (apterous, even-skipped, fushi tarazu, twist, and Rhodopsins 1, 2, 3 and 4) from four Drosophila species (D. erecta, D. pseudoobscura, D. willistoni, and D. littoralis) covering more than 500 kb of the D. melanogaster genome. All D. melanogaster genes (and 78-82% of coding exons) identified in divergent species such as D. pseudoobscura show evidence of functional constraint. Addition of a third species can reveal functional constraint in otherwise non-significant pairwise exon comparisons.

Research   Open Access Highly Accessed

Systematic determination of patterns of gene expression during Drosophila embryogenesis

Pavel Tomancak, Amy Beaton, Richard Weiszmann, Elaine Kwan, ShengQiang Shu, Suzanna E Lewis, Stephen Richards, Michael Ashburner, Volker Hartenstein, Susan E Celniker, Gerald M Rubin Genome Biology 2002, 3:research0088-0088.14 (23 December 2002)

This article is part of a collection on Release 3 of the...

As a first step to creating a comprehensive atlas of gene-expression patterns during Drosophila embryogenesis, 2,179 genes have been examinded by in situ hybridization to fixed Drosophila embryos. Of the genes assayed, 63.7% displayed dynamic expression patterns that were documented with 25,690 digital photomicrographs of individual embryos.

Software   Open Access Highly Accessed

Apollo: a sequence annotation editor

SE Lewis, SMJ Searle, N Harris, M Gibson, V Iyer, J Richter, C Wiel, L Bayraktaroglu, E Birney, MA Crosby, JS Kaminker, BB Matthews, SE Prochnik, CD Smith, JL Tupy, GM Rubin, S Misra, CJ Mungall, ME Clamp Genome Biology 2002, 3:research0082-0082.14 (23 December 2002)

This article is part of a collection on Release 3 of the...

Apollo was developed to enable curators to inspect genome annotations closely and edit them. FlyBase biologists successfully used Apollo to annotate the Drosophila melanogaster genome.

Software   Open Access Highly Accessed

An integrated computational pipeline and database to support whole-genome sequence annotation

CJ Mungall, S Misra, BP Berman, J Carlson, E Frise, N Harris, B Marshall, S Shu, JS Kaminker, SE Prochnik, CD Smith, E Smith, JL Tupy, C Wiel, GM Rubin, SE Lewis Genome Biology 2002, 3:research0081-0081.11 (23 December 2002)

This article is part of a collection on Release 3 of the...

In the course of annotating the Drosophila melanogaster genome sequence, several new open-source software tools and a database schema to support large-scale genome annotation were developed and integrated in to a reusable software system for whole-genome annotation.