Zooming provides additional information. In the top panel, which shows an intermediate level of zoom, the start and stop codons in all three frames appear as green and red tick marks, then the BLASTX similarities to other species (orange bars) and finally BLASTX similarities to Drosophila peptides (red bars). When the level of zoom is increased as shown as the lower panel, the sequence (either nucleic acid or peptide) of any aligned sequences is loaded and displayed to enable curators to directly compare predicted peptides. An individual start or stop codon, such as the start codon shown selected in this example, can be dragged onto the annotation to explicitly specify translation start and end sites for protein coding transcripts in the curator's annotation (blue).
Lewis et al. Genome Biology 2002 3:research0082.1-0082.14 doi:10.1186/gb-2002-3-12-research0082