Genome Biology

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Annotation of the Drosophila melanogaster euchromatic genome: a systematic review

Sima Misra*, Madeline A Crosby, Christopher J Mungall, Beverley B Matthews, Kathryn S Campbell, Pavel Hradecky, Yanmei Huang, Joshua S Kaminker, Gillian H Millburn, Simon E Prochnik, Christopher D Smith, Jonathan L Tupy, Eleanor J Whitfield, Leyla Bayraktaroglu, Benjamin P Berman, Brian R Bettencourt, Susan E Celniker, Aubrey DNJ de Grey, Rachel A Drysdale, Nomi L Harris, John Richter, Susan Russo, Andrew J Schroeder, ShengQiang Shu, Mark Stapleton, Chihiro Yamada, Michael Ashburner, William M Gelbart, Gerald M Rubin and Suzanna E Lewis

Genome Biology 2002, 3:research0083-0083.22 doi:10.1186/gb-2002-3-12-research0083

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BioMed Central: 35 citations

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Hundreds of putatively functional small open reading frames in Drosophila

Emmanuel Ladoukakis, Vini Pereira, Emile G Magny, Adam Eyre-Walker, Juan Pablo Couso Genome Biology 2011, 12:R118 (25 November 2011)

The Drosophila genome contains hundreds of small open reading frames with putative functionality

Research article   Open Access Highly Accessed

Somatic sex-specific transcriptome differences in Drosophila revealed by whole transcriptome sequencing

Peter L Chang, Joseph P Dunham, Sergey V Nuzhdin, Michelle N Arbeitman BMC Genomics 2011, 12:364 (14 July 2011)

Research   Open Access

Synergistic use of plant-prokaryote comparative genomics for functional annotations

Svetlana Gerdes, Basma El Yacoubi, Marc Bailly, Ian K Blaby, Crysten E Blaby-Haas, Linda Jeanguenin, Aurora Lara-Núñez, Anne Pribat, Jeffrey C Waller, Andreas Wilke, Ross Overbeek, Andrew D Hanson, Valérie de Crécy-Lagard BMC Genomics 2011, 12(Suppl 1):S2 (15 June 2011)

Methodology article   Open Access Highly Accessed

TOPSAN: a collaborative annotation environment for structural genomics

Dana Weekes, S Krishna, Constantina Bakolitsa, Ian A Wilson, Adam Godzik, John Wooley BMC Bioinformatics 2010, 11:426 (17 August 2010)

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Regulation of MYCN expression in human neuroblastoma cells

Joannes FM Jacobs, Hans van Bokhoven, Frank N van Leeuwen, Christina A Hulsbergen-van de Kaa, I de Vries, Gosse J Adema, Peter M Hoogerbrugge, Arjan PM de Brouwer BMC Cancer 2009, 9:239 (18 July 2009)

Methodology article   Open Access Highly Accessed

Quantitative measures for the management and comparison of annotated genomes

Karen Eilbeck, Barry Moore, Carson Holt, Mark Yandell BMC Bioinformatics 2009, 10:67 (23 February 2009)

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Non-random retention of protein-coding overlapping genes in Metazoa

Giulia Soldà, Mikita Suyama, Paride Pelucchi, Silvia Boi, Alessandro Guffanti, Ermanno Rizzi, Peer Bork, Maria Tenchini, Francesca D Ciccarelli BMC Genomics 2008, 9:174 (16 April 2008)

Research article   Open Access

Comparative genomic analysis of novel conserved peptide upstream open reading frames in Drosophila melanogaster and other dipteran species

Celine A Hayden, Giovanni Bosco BMC Genomics 2008, 9:61 (1 February 2008)

Conserved peptide upstream open reading frames in Drosophila preferentially associate with transcripts encoding methyltransferases and mitochondrial proteins, suggesting that Drosophila could be used to study the role of these upstream sequences in mitochondrial function and disease.

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Small RNAs and the regulation of cis-natural antisense transcripts in Arabidopsis

Hailing Jin, Vladimir Vacic, Thomas Girke, Stefano Lonardi, Jian-Kang Zhu BMC Molecular Biology 2008, 9:6 (14 January 2008)

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Automated eukaryotic gene structure annotation using EVidenceModeler and the Program to Assemble Spliced Alignments

Brian J Haas, Steven L Salzberg, Wei Zhu, Mihaela Pertea, Jonathan E Allen, Joshua Orvis, Owen White, C Robin Buell, Jennifer R Wortman Genome Biology 2008, 9:R7 (11 January 2008)

EVidenceModeler (EVM) is an automated annotation tool that predicts protein-coding regions, alternatively spliced transcripts and untranslated regions of eukaryotic genes.

Research   Open Access

Fine scale structural variants distinguish the genomes of Drosophila melanogaster and D. pseudoobscura

Stuart J Macdonald, Anthony D Long Genome Biology 2006, 7:R67 (27 July 2006)

Comparative genomics reveals fine-scale structural variants, including microinversions, distinguishing two diverged Drosophila species

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The cnidarian-bilaterian ancestor possessed at least 56 homeoboxes: evidence from the starlet sea anemone, Nematostella vectensis

Joseph F Ryan, Patrick M Burton, Maureen E Mazza, Grace K Kwong, James C Mullikin, John R Finnerty Genome Biology 2006, 7:R64 (24 July 2006)

The first near-complete set of homeodomains from a non-bilaterian animal is described.

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yrGATE: a web-based gene-structure annotation tool for the identification and dissemination of eukaryotic genes

Matthew D Wilkerson, Shannon D Schlueter, Volker Brendel Genome Biology 2006, 7:R58 (19 July 2006)

yrGATE is a new web-based tool for community gene and genome annotation.

Research article   Open Access

Paucity of chimeric gene-transposable element transcripts in the Drosophila melanogaster genome

Mikhail Lipatov, Kapa Lenkov, Dmitri A Petrov, Casey M Bergman BMC Biology 2005, 3:24 (12 November 2005)

Less than 1% of Drosophila transcripts contain 'chimeric' transposable elements, whereas a large proportion of mammalian genomes are known to be derived from TEs; population genetics implies that most fly gene-TE chimeras are deleterious, but a few may contribute to novel gene sequences.

Research article   Open Access

Sequence signature analysis of chromosome identity in three Drosophila species

Per Stenberg, Fredrik Pettersson, Anja O Saura, Anders Berglund, Jan Larsson BMC Bioinformatics 2005, 6:158 (23 June 2005)

Research article   Open Access

Conserved genomic organisation of Group B Sox genes in insects.

Carol McKimmie, Gertrud Woerfel, Steven Russell BMC Genetics 2005, 6:26 (19 May 2005)

Research article   Open Access

Complete reannotation of the Arabidopsis genome: methods, tools, protocols and the final release

Brian J Haas, Jennifer R Wortman, Catherine M Ronning, Linda I Hannick, Roger K Smith, Rama Maiti, Agnes P Chan, Chunhui Yu, Maryam Farzad, Dongying Wu, Owen White, Christopher D Town BMC Biology 2005, 3:7 (22 March 2005)

The TIGR group explain how they reannotated the Arabidopsis genome, with several thousand new genes and pseudogenes annotated, 1/3 of the original annotations refined and every protein-coding gene assigned to a Gene Ontology term.

Research   Open Access

Comparison of the oxidative phosphorylation (OXPHOS) nuclear genes in the genomes of Drosophila melanogaster, Drosophila pseudoobscura and Anopheles gambiae

Gaetano Tripoli, Domenica D'Elia, Paolo Barsanti, Corrado Caggese Genome Biology 2005, 6:R11 (31 January 2005)

An analysis of nuclear-encoded oxidative phosphorylation genes in Drosophila and Anopheles reveals that pairs of duplicated genes have strikingly different expression patterns.

Database   Open Access

DEDB: a database of Drosophila melanogaster exons in splicing graph form

Bernett TK Lee, Tin Tan, Shoba Ranganathan BMC Bioinformatics 2004, 5:189 (7 December 2004)

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The regulatory content of intergenic DNA shapes genome architecture

Craig E Nelson, Bradley M Hersh, Sean B Carroll Genome Biology 2004, 5:R25 (15 March 2004)

The relationship between regulatory complexity and gene spacing was examined in Caenorhabditis elegans and Drosophila melanogaster. Intergenic distance, and hence genome architecture, is shaped by regulatory information contained in noncoding DNA.

Method   Open Access

FlyGEM, a full transcriptome array platform for the Drosophila community

Rick Johnston, Bruce Wang, Rachel Nuttall, Michael Doctolero, Pamela Edwards, Jining Lü, Marina Vainer, Huibin Yue, Xinhao Wang, James Minor, Cathy Chan, Alex Lash, Thomas Goralski, Michael Parisi, Brian Oliver, Scott Eastman Genome Biology 2004, 5:R19 (26 February 2004)

An array, FlyGEM, has been constructed based on amplification of exons from Drosophila genomic DNA. The array gene list has been extensively annotated and linked to other databases.

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A comprehensive evolutionary classification of proteins encoded in complete eukaryotic genomes

Eugene V Koonin, Natalie D Fedorova, John D Jackson, Aviva R Jacobs, Dmitri M Krylov, Kira S Makarova, Raja Mazumder, Sergei L Mekhedov, Anastasia N Nikolskaya, B Rao, Igor B Rogozin, Sergei Smirnov, Alexander V Sorokin, Alexander V Sverdlov, Sona Vasudevan, Yuri I Wolf, Jodie J Yin, Darren A Natale Genome Biology 2004, 5:R7 (15 January 2004)

We examined functional and evolutionary patterns in the recently constructed set of 5,873 clusters of predicted orthologs from seven eukaryotic genomes. The analysis reveals a conserved core of largely essential eukaryotic genes as well as major diversification and innovation associated with evolution of eukaryotic genomes.

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How many genes in a genome?

Brian Oliver, Benoit Leblanc Genome Biology 2003, 5:204 (22 December 2003)

A recent study of the Drosophila genome has added perhaps 2,000 genes to the predicted total, and raises a number of questions about how genome annotation data should be stored and presented.

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An integrated gene annotation and transcriptional profiling approach towards the full gene content of the Drosophila genome

M Hild, B Beckmann, SA Haas, B Koch, V Solovyev, C Busold, K Fellenberg, M Boutros, M Vingron, F Sauer, JD Hoheisel, R Paro Genome Biology 2003, 5:R3 (22 December 2003)

A novel Drosophila microarray constructed on the basis of an integrated in silico/wet biology approach provides evidence for the transcription of approximately 2,600 additional genes. Validation indicates a lower limit of 2,000 novel annotations, thus raising the number of genes that make a fly.

Research article   Open Access

Genomic characterization of a repetitive motif strongly associated with developmental genes in Drosophila

Javier Costas, Cristina P Vieira, Fernando Casares, Jorge Vieira BMC Genomics 2003, 4:52 (16 December 2003)

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Computational identification of Drosophila microRNA genes

Eric C Lai, Pavel Tomancak, Robert W Williams, Gerald M Rubin Genome Biology 2003, 4:R42 (30 June 2003)

An informatic procedure has been used to analyze the euchromatic sequences of Drosophila melanogaster and D. pseudoobscura for conserved sequences that adopt an extended stem-loop structure and display other characteristics of known miRNAs.

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Impressive expressions: developing a systematic database of gene-expression patterns in Drosophila embryogenesis

Haiqiong Montalta-He, Heinrich Reichert Genome Biology 2003, 4:205 (28 January 2003)

The establishment of a database of gene-expression patterns derived from systematic highthroughput in situ hybridization studies on whole-mount Drosophila embryos vastly increases the breadth and depth that can be reached by developmental genetics.

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Heterochromatic sequences in a Drosophila whole-genome shotgun assembly

Roger A Hoskins, Christopher D Smith, Joseph W Carlson, A Bernardo Carvalho, Aaron Halpern, Joshua S Kaminker, Cameron Kennedy, Chris J Mungall, Beth A Sullivan, Granger G Sutton, Jiro C Yasuhara, Barbara T Wakimoto, Eugene W Myers, Susan E Celniker, Gerald M Rubin, Gary H Karpen Genome Biology 2002, 3:research0085-0085.16 (31 December 2002)

This article is part of a collection on Release 3 of the...

Annotation of an improved whole-genome shotgun assembly of the Drosophila melanogaster genome predicted 297 protein-coding genes and six non-protein-coding genes, including known heterochromatic genes, and regions of similarity to known transposable elements. Fluorescence in situ hybridization was used to correlate the genomic sequence with the cytogenetic map; the annotated euchromatic sequence extends into the centric heterochromatin on each chromosome arm.

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Assessing the impact of comparative genomic sequence data on the functional annotation of the Drosophila genome

Casey M Bergman, Barret D Pfeiffer, Diego E Rincón-Limas, Roger A Hoskins, Andreas Gnirke, Chris J Mungall, Adrienne M Wang, Brent Kronmiller, Joanne Pacleb, Soo Park, Mark Stapleton, Kenneth Wan, Reed A George, Pieter J de Jong, Juan Botas, Gerald M Rubin, Susan E Celniker Genome Biology 2002, 3:research0086-0086.20 (30 December 2002)

This article is part of a collection on Release 3 of the...

Analysis of conservation in eight genomic regions (apterous, even-skipped, fushi tarazu, twist, and Rhodopsins 1, 2, 3 and 4) from four Drosophila species (D. erecta, D. pseudoobscura, D. willistoni, and D. littoralis) covering more than 500 kb of the D. melanogaster genome. All D. melanogaster genes (and 78-82% of coding exons) identified in divergent species such as D. pseudoobscura show evidence of functional constraint. Addition of a third species can reveal functional constraint in otherwise non-significant pairwise exon comparisons.

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The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective

Joshua S Kaminker, Casey M Bergman, Brent Kronmiller, Joseph Carlson, Robert Svirskas, Sandeep Patel, Erwin Frise, David A Wheeler, Suzanna E Lewis, Gerald M Rubin, Michael Ashburner, Susan E Celniker Genome Biology 2002, 3:research0084-0084.20 (23 December 2002)

This article is part of a collection on Release 3 of the...

Using Release 3 of the euchromatic genomic sequence of Drosophila melanogaster, 85 known and eight novel families of transposable element have been identified, varying in copy number from one to 146. A total of 1,572 full and partial transposable elements were identified, comprising 3.86% of the sequence.

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Apollo: a sequence annotation editor

SE Lewis, SMJ Searle, N Harris, M Gibson, V Iyer, J Richter, C Wiel, L Bayraktaroglu, E Birney, MA Crosby, JS Kaminker, BB Matthews, SE Prochnik, CD Smith, JL Tupy, GM Rubin, S Misra, CJ Mungall, ME Clamp Genome Biology 2002, 3:research0082-0082.14 (23 December 2002)

This article is part of a collection on Release 3 of the...

Apollo was developed to enable curators to inspect genome annotations closely and edit them. FlyBase biologists successfully used Apollo to annotate the Drosophila melanogaster genome.

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An integrated computational pipeline and database to support whole-genome sequence annotation

CJ Mungall, S Misra, BP Berman, J Carlson, E Frise, N Harris, B Marshall, S Shu, JS Kaminker, SE Prochnik, CD Smith, E Smith, JL Tupy, C Wiel, GM Rubin, SE Lewis Genome Biology 2002, 3:research0081-0081.11 (23 December 2002)

This article is part of a collection on Release 3 of the...

In the course of annotating the Drosophila melanogaster genome sequence, several new open-source software tools and a database schema to support large-scale genome annotation were developed and integrated in to a reusable software system for whole-genome annotation.

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A Drosophila full-length cDNA resource

Mark Stapleton, Joe Carlson, Peter Brokstein, Charles Yu, Mark Champe, Reed George, Hannibal Guarin, Brent Kronmiller, Joanne Pacleb, Soo Park, Ken Wan, Gerald M Rubin, Susan E Celniker Genome Biology 2002, 3:research0080-0080.8 (23 December 2002)

This article is part of a collection on Release 3 of the...

High-quality full-insert sequence for 8,921 putative full-length cDNA clones in the Drosophila Gene Collection has been generated and compared to the annotated Release 3 genomic sequence. More than 5,300 cDNAs have been identifieed that contain a complete and accurate protein-coding sequence, corresponding to at least one splice form for 40% of the predicted D. melanogaster genes.

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Finishing a whole-genome shotgun: Release 3 of the Drosophila melanogaster euchromatic genome sequence

Susan E Celniker, David A Wheeler, Brent Kronmiller, Joseph W Carlson, Aaron Halpern, Sandeep Patel, Mark Adams, Mark Champe, Shannon P Dugan, Erwin Frise, Ann Hodgson, Reed A George, Roger A Hoskins, Todd Laverty, Donna M Muzny, Catherine R Nelson, Joanne M Pacleb, Soo Park, Barret D Pfeiffer, Stephen Richards, Erica J Sodergren, Robert Svirskas, Paul E Tabor, Kenneth Wan, Mark Stapleton, Granger G Sutton, Craig Venter, George Weinstock, Steven E Scherer, Eugene W Myers, Richard A Gibbs, Gerald M Rubin et al. Genome Biology 2002, 3:research0079-0079.14 (23 December 2002)

This article is part of a collection on Release 3 of the...

The Drosophila melanogaster genome was the first metazoan genome to be sequenced by whole-genome shotgun. Now, the sequence has been finished in a process designed to close gaps, improve sequence quality and validate the assembly.

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Computational analysis of core promoters in the Drosophila genome

Uwe Ohler, Guo-chun Liao, Heinrich Niemann, Gerald M Rubin Genome Biology 2002, 3:research0087-0087.12 (20 December 2002)

This article is part of a collection on Release 3 of the...

Candidate transcription start sites have been identified for about 2,000 Drosophila genes by aligning 5' expressed sequence tags (ESTs) from cap-trapped cDNA libraries to the genome. Examination of the sequences flanking these candidate transcription start sites revealed the presence of well-known core promoter motifs such as the TATA box, the initiator and the downstream promoter element (DPE).