Figure 4.

Evidence supporting the gene models. The diagram shows the numbers of curated gene models supported by evidence in three classes. Sequence alignments to Drosophila ESTs and, in parentheses, full-insert cDNA sequences were determined using sim4 (yellow circle). Gene predictions were made using Genie and Genscan (red circle). Similarities to known and predicted genes and proteins in Drosophila and other organisms were determined using BLASTX and TBLASTX (blue circle). The intersections in the diagram show the number of gene models that are supported by multiple evidence types. For example, there are 80 models supported by all three types of evidence, and 46 of these are represented by full-insert cDNA sequence. The 89 gene models supported only by gene prediction include 22 models that were predicted only in the masked sequence.