Table 1

Identity of genes differentially expressed between naive and experienced honeybees and in caffeine-treated honeybees


Array data





bEST number
 Gene
 Head (foragers vs naive)
 Abdomen (foragers vs naive)
 Head (caffeine treated vs control)
 Predicted function
 Similarity to closest relative (%)
5
 Hsc70
 -1.6
 +1.3
 +12.6
 Chaperone, heat shock response, neurotransmitter release
 95%
22
 α-Gluc-1
 +2.5
 -1.1
 +2.2
 α-Glucosidase
 100%
28
 Unknown
 -3.2
 +2.3
 -1.5
 Unknown
 N/A
30
 Hsp20
 +1.3
 +2.1
 -1.1
 Chaperone
 65%
31
 Hsp83
 -1.6
 +2.4
 +1.2
 Chaperone, heat shock response, protein folding
 92%
36
 SPARC
 -8.2
 -3.5
 +1.8
 Cell adhesion, calcium binding
 61%
54
 GPDH 2
 -3.1
 +1.3
 -1.1
 Mitochondrial glycerol-3-phosphate dehydrogenase
 69%
56
 Cox10
 -4.0
 -1.1
 -1.2
 Protoheme IX farnesyltransferase, cox assembly
 76%
57
 Stck
 -2.6
 -1.5
 +3.9
 LIM-domain protein, transcriptional regulation
 88%
61
 Unknown
 -5.2
 -1.3
 +1.4
 PDZ-domain protein, signal transduction
 50%
82
 Aminopeptidase-1
 +1.6
 +1.0
 -1.2
 Membrane alanyl aminopeptidase
 51%
92
 Trf
 +1.2
 +3.0
 +2.8
 Iron transport, iron homeostasis, defense response
 63%
97
 CG16857
 -3.3
 +2.1
 +1.5
 Ig-domain protein, cell adhesion
 63%
99
 RpS19
 -4.7
 -1.7
 -1.6
 Structural protein of ribosome, protein biosynthesis
 83%
102
 MRJP2
 +12.1
 +1.7
 -1.3
 Royal-jelly protein
 100%
105
 Gld
 +3.0
 -1.0
 +2.0
 Glucose dehydrogenase
 100%
108
 PPIase
 -4.0
 -1.1
 -1.5
 Peptidylprolyl isomerase
 92%
109
 ATP synthase β
 -5.9
 -1.4
 +1.6
 Hydrogen-transporting two-sector ATPase
 95%
112
 Unknown
 +5.5
 -1.1
 -1.3
 Unknown
 N/A
121
 IDGF
 -1.4
 +1.9
 +2.4
 Imaginal disc growth factor, not chitinase
 54%
122
 α-Glucosidase-2
 -2.3
 +9.8
 +1.2
 α-Glucosidase
 60%
123
 Unknown
 -3.3
 -1.1
 -1.7
 Unknown
 N/A
124
 Peritrophin
 -5.6
 -4.3
 +1.4
 Structural protein of peritrophic membrane, chitin binding
 78%
125
 Hymenoptaecin
 +1.5
 +8.0
 +1.7
 Antibacterial protein, defense response
 100%
127
 CG6112
 -7.1
 -3.8
 -1.3
 Ligand-gated ion channel subunit
 98%
128
 Aminopeptidase-2
 -1.4
 -1.2
 +1.5
 Membrane alanyl aminopeptidase
 49%
129
 Unknown
 -1.9
 +1.2
 +5.6
 Unknown
 N/A
130
 Unknown
 -7.2
 -1.7
 -1.1
 Unknown
 N/A
131
 Lectin
 -16.0
 -5.9
 -1.3
 Ligand binding or carrier
 82%
132
 CoxI
 -4.9
 +1.0
 +1.1
 Respiratory-chain enzyme
 100%
133
 Scp1
 -2.4
 -1.2
 -1.7
 Small chemosensory protein
 74%
134
 ATPsynthase F0-6
 -14.1
 -1.4
 -1.0
 Proton pump, ATP synthesis
 100%
136
 Unknown
 N/A
 +1.2
 +4.3
 Unknown
 N/A
138
 Gs2
 N/A
 +1.8
 +3.5
 Glutamine synthase
 77%
143
 Unknown
 N/A
 -1.0
 +3.4
 Unknown
 N/A
147
 CG5586
 N/A
 -1.3
 -3.2
 WD40 and SOCS domain-containing protein
 38%
148
 Aminotransferase
 -2.6
 -1.4
 -3.5
 Ornithine-oxo-acid aminotransferase
 73%

bEST number, cDNA number; gene, gene identifier; array data, fold change calculated from array hybridization data using pairwise comparisons for heads, abdomens and caffeine-treated bees; predicted function, putative function inferred from sequence similarity; Percentage similarity to the closest relative in GenBank. Accession numbers of bESTs reported in this paper: 5, BI946410; 22, BI946425; 28, BI946431; 30, BI946433; 31, BI946435; 36, BI946440; 54, BI946454; 56, BI946456; 57, BI946458; 61, BI946461; 82, BI946480; 92, BI946487; 97, BI946490; 99, BI946493; mrjp2 (102), af000632; gld (105), ab022907; 108, BI946499; 109, BI946500; 112, BI946503; 121, BI946511; 122, BI946512; 123, BI946513; 124, BI946514; hymenoptaecin (125), amu15956; 127, BI946517; 128, BI946519; 129, BI946520; 130, BI946522; 131, BI946524; 132, BI946525; 133, BI946526; 134, BI946527; 136, BI946528; 138, BI946532; 143, BI946537; 147, BI946542; 148, BI946543.

Kucharski and Maleszka Genome Biology 2002 3:research0007.1   doi:10.1186/gb-2002-3-2-research0007

Open Data