Table 1 |
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|
Identity of genes differentially expressed between naive and experienced honeybees and in caffeine-treated honeybees |
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Array data |
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|
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|
bEST number |
Gene |
Head (foragers vs naive) |
Abdomen (foragers vs naive) |
Head (caffeine treated vs control) |
Predicted function |
Similarity to closest relative (%) |
|
|
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|
5 |
Hsc70 |
-1.6 |
+1.3 |
+12.6 |
Chaperone, heat shock response, neurotransmitter release |
95% |
|
22 |
α-Gluc-1 |
+2.5 |
-1.1 |
+2.2 |
α-Glucosidase |
100% |
|
28 |
Unknown |
-3.2 |
+2.3 |
-1.5 |
Unknown |
N/A |
|
30 |
Hsp20 |
+1.3 |
+2.1 |
-1.1 |
Chaperone |
65% |
|
31 |
Hsp83 |
-1.6 |
+2.4 |
+1.2 |
Chaperone, heat shock response, protein folding |
92% |
|
36 |
SPARC |
-8.2 |
-3.5 |
+1.8 |
Cell adhesion, calcium binding |
61% |
|
54 |
GPDH 2 |
-3.1 |
+1.3 |
-1.1 |
Mitochondrial glycerol-3-phosphate dehydrogenase |
69% |
|
56 |
Cox10 |
-4.0 |
-1.1 |
-1.2 |
Protoheme IX farnesyltransferase, cox assembly |
76% |
|
57 |
Stck |
-2.6 |
-1.5 |
+3.9 |
LIM-domain protein, transcriptional regulation |
88% |
|
61 |
Unknown |
-5.2 |
-1.3 |
+1.4 |
PDZ-domain protein, signal transduction |
50% |
|
82 |
Aminopeptidase-1 |
+1.6 |
+1.0 |
-1.2 |
Membrane alanyl aminopeptidase |
51% |
|
92 |
Trf |
+1.2 |
+3.0 |
+2.8 |
Iron transport, iron homeostasis, defense response |
63% |
|
97 |
CG16857 |
-3.3 |
+2.1 |
+1.5 |
Ig-domain protein, cell adhesion |
63% |
|
99 |
RpS19 |
-4.7 |
-1.7 |
-1.6 |
Structural protein of ribosome, protein biosynthesis |
83% |
|
102 |
MRJP2 |
+12.1 |
+1.7 |
-1.3 |
Royal-jelly protein |
100% |
|
105 |
Gld |
+3.0 |
-1.0 |
+2.0 |
Glucose dehydrogenase |
100% |
|
108 |
PPIase |
-4.0 |
-1.1 |
-1.5 |
Peptidylprolyl isomerase |
92% |
|
109 |
ATP synthase β |
-5.9 |
-1.4 |
+1.6 |
Hydrogen-transporting two-sector ATPase |
95% |
|
112 |
Unknown |
+5.5 |
-1.1 |
-1.3 |
Unknown |
N/A |
|
121 |
IDGF |
-1.4 |
+1.9 |
+2.4 |
Imaginal disc growth factor, not chitinase |
54% |
|
122 |
α-Glucosidase-2 |
-2.3 |
+9.8 |
+1.2 |
α-Glucosidase |
60% |
|
123 |
Unknown |
-3.3 |
-1.1 |
-1.7 |
Unknown |
N/A |
|
124 |
Peritrophin |
-5.6 |
-4.3 |
+1.4 |
Structural protein of peritrophic membrane, chitin binding |
78% |
|
125 |
Hymenoptaecin |
+1.5 |
+8.0 |
+1.7 |
Antibacterial protein, defense response |
100% |
|
127 |
CG6112 |
-7.1 |
-3.8 |
-1.3 |
Ligand-gated ion channel subunit |
98% |
|
128 |
Aminopeptidase-2 |
-1.4 |
-1.2 |
+1.5 |
Membrane alanyl aminopeptidase |
49% |
|
129 |
Unknown |
-1.9 |
+1.2 |
+5.6 |
Unknown |
N/A |
|
130 |
Unknown |
-7.2 |
-1.7 |
-1.1 |
Unknown |
N/A |
|
131 |
Lectin |
-16.0 |
-5.9 |
-1.3 |
Ligand binding or carrier |
82% |
|
132 |
CoxI |
-4.9 |
+1.0 |
+1.1 |
Respiratory-chain enzyme |
100% |
|
133 |
Scp1 |
-2.4 |
-1.2 |
-1.7 |
Small chemosensory protein |
74% |
|
134 |
ATPsynthase F0-6 |
-14.1 |
-1.4 |
-1.0 |
Proton pump, ATP synthesis |
100% |
|
136 |
Unknown |
N/A |
+1.2 |
+4.3 |
Unknown |
N/A |
|
138 |
Gs2 |
N/A |
+1.8 |
+3.5 |
Glutamine synthase |
77% |
|
143 |
Unknown |
N/A |
-1.0 |
+3.4 |
Unknown |
N/A |
|
147 |
CG5586 |
N/A |
-1.3 |
-3.2 |
WD40 and SOCS domain-containing protein |
38% |
|
148 |
Aminotransferase |
-2.6 |
-1.4 |
-3.5 |
Ornithine-oxo-acid aminotransferase |
73% |
|
|
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|
bEST number, cDNA number; gene, gene identifier; array data, fold change calculated from array hybridization data using pairwise comparisons for heads, abdomens and caffeine-treated bees; predicted function, putative function inferred from sequence similarity; Percentage similarity to the closest relative in GenBank. Accession numbers of bESTs reported in this paper: 5, BI946410; 22, BI946425; 28, BI946431; 30, BI946433; 31, BI946435; 36, BI946440; 54, BI946454; 56, BI946456; 57, BI946458; 61, BI946461; 82, BI946480; 92, BI946487; 97, BI946490; 99, BI946493; mrjp2 (102), af000632; gld (105), ab022907; 108, BI946499; 109, BI946500; 112, BI946503; 121, BI946511; 122, BI946512; 123, BI946513; 124, BI946514; hymenoptaecin (125), amu15956; 127, BI946517; 128, BI946519; 129, BI946520; 130, BI946522; 131, BI946524; 132, BI946525; 133, BI946526; 134, BI946527; 136, BI946528; 138, BI946532; 143, BI946537; 147, BI946542; 148, BI946543. |
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|
Kucharski and Maleszka Genome Biology 2002 3:research0007.1 doi:10.1186/gb-2002-3-2-research0007 |
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