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Vasculature mapping

Jonathan B Weitzman

Author Affiliations

Genome Biology 2002, 3:spotlight-20020213-02  doi:10.1186/gb-spotlight-20020213-02


The electronic version of this article is the complete one and can be found online at:


Published:13 February 2002

© 2002 BioMed Central Ltd

Research news

A few years ago Renata Pasqualini and Erkki Ruoslahti developed an in vivo selection method to identify peptides that target specific vascular beds following intravenous administration of random peptide phage-display libraries in mice. In the February issue of Nature Medicine, Pasqualini's group at the MD Anderson Cancer Center in Texas report the application of 'in vivo phage display' to characterize the human vasculature (Nature Medicine 2002, 8:121-127). They injected a large-scale, random peptide library into a terminally ill patient and collected tissue samples 15 minutes post-infusion. They recovered phage from different organs and carried out a high-throughput analysis of over 4,000 phage inserts. Comparison of the selected tripeptide motif frequencies revealed a non-random, tissue-specific distribution. They then used a phage-overlay histological assay to validate peptide sequences specific for prostate or skin tissues. This study represents a first step towards a well-characterized map of the heterogeneity within the human vasculatuure, opening up the possibilities for targeted drug delivery strategies.

References

  1. Organ targeting in vivo using phage display peptide libraries.

    PubMed Abstract OpenURL

  2. [http://medicine.nature.com] webcite

    Nature Medicine

  3. [http://www.mdanderson.org] webcite

    MD Anderson Cancer Center