Table 3 |
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|
Comparison of change folds between oligonucleotide arrays and RT-PCR |
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|
Change fold |
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|
|
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|
Avg Diff |
hsotd |
hsOtx2 |
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|
|
|
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|
Transcript |
hswt |
hsotd |
hsOtx2 |
Array |
RT-PCR |
Array |
RT-PCR |
|
|
|||||||
|
scra |
251 |
1375 |
1229 |
5.5 |
1.3 |
4.9 |
1.6 |
|
LysD |
525 |
1646 |
2436 |
3.1 |
1.6 |
4.6 |
4.0 |
|
glu |
479 |
1196 |
1991 |
2.5 |
1.8 |
4.2 |
10.9 |
|
Rpd3 |
1170 |
2562 |
2673 |
2.2 |
2.0 |
2.3 |
2.5 |
|
pim |
118 |
246 |
1467 |
2.1 |
1.4 |
12.4 |
8.0 |
|
n-syb |
612 |
293 |
296 |
-2.1 |
-1.5 |
-2.1 |
-1.5 |
|
eyg |
1552 |
229 |
10 |
-6.7 |
-1.4 |
-77.6 |
-1.6 |
|
wun |
885 |
/ |
884 |
/ |
/ |
1.0 |
1.0 |
|
Scc1 |
724 |
723 |
/ |
1.0 |
1.0 |
/ |
/ |
|
otd |
84 |
6555 |
108 |
78.0 |
119.4 |
1.3 |
1.5 |
|
|
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|
RT-PCR was carried out on cDNA derived from heat-shocked wild type (hswt), heat-shocked otd (hsotd) or heat-shocked Otx2 (hsOtx2) embryos. Change folds determined by RT-PCR are represented as the mean value of eight independent replicates, derived from two different cDNA preparations. wun is used as a control for the comparison of the RT-PCR data between heat-shocked wild type and heat-shocked Otx2. Scc1 is used as a control for the comparison of the RT-PCR data between heat-shocked wild type and heat-shocked otd. |
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|
Montalta-He et al. Genome Biology 2002 3:research0015.1 doi:10.1186/gb-2002-3-4-research0015 |
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