Table 1

Predicted attenuators in the genome of B. subtilis

ID
Z-score
Known
Upstream promotera
Geneb

BS0929
-8.895


glycerol-3-phosphate dehydrogenase (glpD)
BS2825
-8.473


acetolactate synthase (acetohydroxy-acid synthase) (large subunit) (ilvB)
BS1310
-6.836


similar to chaperonin (ykkC)
BS0983
-6.733


hypothetical protein (yhaW)
BS3920
-6.468


PTS beta-glucoside-specific enzyme IIABC component (bglP)
BS1257
-6.332


RNA polymerase PBSX sigma factor-like (xpf)
BS2733
-6.238


alanyl-tRNA synthetase (alaS)
BS0013
-6.126


seryl-tRNA synthetase (serS)
BS2184
-5.942


hypothetical protein (yphP)
BS3966
-5.840


hypothetical protein (iolD)
BS2749
-5.839


histidyl-tRNA synthetase (hisS)
BS2520
-5.815


glycyl-tRNA synthetase (alpha subunit) (glyQ)
BS3396
-5.767


similar to amino acid permease (yvbW)
BS0215
-5.526


glycerol-3-phosphate permease (glpT)
BS2255
-5.344


hypothetical protein (ypiA)
BS2600
-5.342


similar to phage-related protein (yqbK)
BS1544
-5.239


isoleucyl-tRNA synthetase (ileS)
BS3710
-5.196


CTP synthetase (ctrA)
BS3895
-5.191


similar to pyrimidine nucleoside transport protein (yxjA)
BS3798
-5.164


PTS sucrose-specific enzyme IIBC component (sacP)
BS2614
-5.152


hypothetical protein (yqaQ)
BS2484
-5.148


similar to 5-formyltetrahydrofolate cyclo-ligase (yqgN)
BS1319
-5.124


cobalamin-independent methionine synthase (metC)
BS2682
-4.990


hypothetical protein (yraL)
BS3750
-4.958


threonyl-tRNA synthetase (thrZ)
BS0183
-4.938


NADH dehydrogenase (subunit 5) (ndhF)
BS3440
-4.890


levansucrase (sacB)
BS2715
-4.841


similar to formate dehydrogenase (yrhE)
BS2204
-4.819


xanthine phosphoribosyltransferase (xpt)
BS2139
-4.756


similar to N-acetylmuramoyl-L-alanine amidase (yomC)
BS2803
-4.750


valyl-tRNA synthetase (valS)
BS0093
-4.747


serine acetyltransferase (cysE)
BS3877
-4.696


hypothetical protein (yxkD)
BS1357
-4.680


hypothetical protein (ykrT)
BS0178
-4.675


L-glutamine-D-fructose-6-phosphate amidotransferase (glmS)
BS1658
-4.644


DNA polymerase III (alpha subunit) (polC)
BS3894
-4.630


hypothetical protein (yxjB)
tRNA-Arg
-4.609


tRNA-Arg (trnJ-Arg)
BS0535
-4.505


similar to antibiotic resistance protein (ydfB)
BS3839
-4.472


tyrosyl-tRNA synthetase (tyrZ)
BS2961
-4.430


tyrosyl-tRNA synthetase (tyrS)
BS0441
-4.399


hypothetical protein (ydbB)
BS1143
-4.382


tryptophanyl-tRNA synthetase (trpS)
BS1737
-4.375


similar to ribonucleoprotein (ymaA)
BS0254
-4.309


hypothetical protein (yczA)
BS1971
-4.297


similar to adenosylmethionine-8-amino-7-oxononanoate aminotransferase (yodT)
BS0643
-4.279


phosphoribosylaminoimidazole carboxylase I (purE)
BS3026
-4.270


leucyl-tRNA synthetase (leuS)
BS2889
-4.249


threonyl-tRNA synthetase (thrS)
BS3690
-4.191


hypothetical protein (ywlC)
BS1188
-4.176


similar to cystathionine gamma-synthase (yjcI)
BS0521
-4.158


hypothetical protein (ydeI)
BS2452
-4.143


similar to aminomethyltransferase (yqhI)
BS3604
-4.128


hypothetical protein (ywrE)
BS1313
-4.086


gamma-glutamyl kinase (proB)
BS3269
-3.989


similar to ABC transporter (ATP-binding protein) (yusC)
BS0423
-3.974


hypothetical protein (ydaH)
BS2504
-3.896


similar to transcriptional regulator (Fur family) (yqfV)
BS3900
-3.884


endo-beta-1,3-1,4 glucanase (bglS)
BS0038
-3.883


methionyl-tRNA synthetase (metS)
BS2319
-3.879


hypothetical protein (ypuF)
BS1683
-3.845


similar to phage-related protein (ymfE)
BS1881
-3.805


phosphoenolpyruvate synthase (pps)
BS3513
-3.805


putative membrane protein (csbA)
BS0432
-3.796


hypothetical protein (ydaO)
BS3355
-3.795


hypothetical protein (yvaI)
BS2858
-3.784


phenylalanyl-tRNA synthetase (alpha subunit) (pheS)
BS3668
-3.780


hypothetical protein (ywmD)
BS0927
-3.776


glycerol uptake facilitator (glpF)
BS0281
-3.739


hypothetical protein (ycdC)
BS2306
-3.714


RNA polymerase ECF-type sigma factor (sigma-X) (sigX)
BS0143
-3.709


RNA polymerase (alpha subunit) (rpoA)
BS0449
-3.687


similar to ABC transporter (ATP-binding protein) (ydbJ)
BS1094
-3.668


hypothetical protein (yitC)
BS1370
-3.645


motility protein A (motA)
BS3785
-3.618


hypothetical protein (ywdL)
BS0104
-3.612


ribosomal protein L10 (BL5) (rplJ)
BS3313
-3.584


similar to iron-binding protein (yvrC)
BS0716
-3.572


hypothetical protein (yetH)
BS1889
-3.524


response regulator aspartate phosphatase (rapK)
BS0673
-3.492


hypothetical protein (yerQ)
BS0002
-3.480


DNA polymerase III (beta subunit) (dnaN)
BS0880
-3.473


transcriptional regulator (senS)
BS1548
-3.473


transcriptional attenuator and uracil phosphoribosyltransferase activity (minor) (pyrR)
BS1409
-3.467


hypothetical protein (ykuH)
BS2881
-3.464


initiation factor IF-3 (infC)
BS1651
-3.412


uridylate kinase (smbA)
BS3888
-3.412


hypothetical protein (yxjH)
BS3889
-3.412


hypothetical protein (yxjG)
BS0925
-3.407


similar to adenosylmethionine-8-amino-7-oxononanoate aminotransferase (yhxA)
BS1205
-3.351


hypothetical protein (yjdG)
BS3784
-3.335


hypothetical protein (spsA)
BS3093
-3.325


hypothetical protein (yuaJ)
BS1470
-3.265


hypothetical protein (yktD)
BS1549
-3.265


uracil permease (pyrP)
BS2841
-3.262


aspartokinase II (lysC)
BS2903
-3.222


DNA polymerase I (polA)
BS3446
-3.202


hypothetical protein (yvdQ)
BS2984
-3.202


hypothetical protein (ytmQ)
BS1920
-3.201


similar to ATP-dependent DNA helicase (yocI)
BS3343
-3.192


hypothetical protein (yvgV)
BS3731
-3.192


hypothetical protein (ywiA)
BS3278
-3.188


similar to 3-hydroxyacyl-CoA dehydrogenase (yusL)
BS3412
-3.180


transcriptional regulator (LacI family) (lacR)
BS2278
-3.156


hypothetical protein (yphE)
BS3914
-3.141


hypothetical protein (yxiF)
BS1550
-3.120


aspartate carbamoyltransferase (pyrB)
BS0878
-3.120


thiamin biosynthesis protein (thiA)
BS2775
-3.111


similar to sodium/proton-dependent alanine carrier protein (yrbD)
BS1673
-3.111


dipicolinate synthase subunit A (spoVFA)
BS2569
-3.086


site-specific DNA recombinase (spoIVCA)
BS2713
-3.075


similar to formate dehydrogenase (yrhG)
BS2539
-3.075


heat-shock protein (dnaJ)
BS3719
-3.068


similar to cardiolipin synthetase (ywiE)
BS2711
-3.061


similar to methyltransferase (yrhH)
BS1166
-3.059


transcriptional regulator (tenA)
BS1446
-3.057


aminopeptidase (ampS)
BS0503
-3.033


hypothetical protein (yddM)
BS1455
-3.014


hypothetical protein (ykzG)
BS3164
-2.969


transcriptional regulator (comQ)
BS1360
-2.966


similar to ribulose-bisphosphate carboxylase (ykrW)
BS1536
-2.942


similar to acetylornithine deacetylase (ylmB)
BS3133
-2.940


similar to polyribonucleotide nucleotidyltransferase (yugI)
BS3635
-2.930


flagellar basal-body rod protein (flhO)
BS1659
-2.908


hypothetical protein (ylxS)
BS2216
-2.896


hypothetical protein (ypsA)
BS0115
-2.894


ribosomal protein S10 (BS13) (rpsJ)
BS0637
-2.878


hypothetical protein (yebB)
BS2627
-2.867


similar to transcriptional regulator (phage-related) (Xre family) (yqaE)
BS0745
-2.856


similar to quinone oxidoreductase (yfmJ)
BS1785
-2.852


transcriptional regulator (lexA)
BS1737
-2.805


similar to ribonucleoprotein (ymaA)
BS1525
-2.800


cell-division initiation protein (divIB)
BS2138
-2.782


hypothetical protein (yomD)
BS2162
-2.776


hypothetical protein (yokC)
BS1335
-2.740


similar to transcriptional regulator (MarR family) (ykoM)
BS0241
-2.658


similar to histidine permease (ybgF)
BS2715
-2.649


similar to formate dehydrogenase (yrhE)
BS1855
-2.649


similar to phosphoglycerate dehydrogenase (yoaD)
BS2986
-2.643


hypothetical protein (ytmP)
BS1321
-2.642


hypothetical protein (ispU)
BS0386
-2.640


similar to transcriptional regulator (TetR/AcrR family) (ycnC)
BS2425
-2.616


similar to exodeoxyribonuclease VII (large subunit) (yqiB)
BS0057
-2.589


similar to amino acid transporter (yabM)
BS3957
-2.586


similar to ABC transporter (ATP-binding protein) (yxdL)
BS1003
-2.585


Hit-like protein (hit)
BS3350
-2.580


hypothetical protein (yvaC)
BS1659
-2.573


hypothetical protein (ylxS)
BS4050
-2.567


hypothetical protein (yybP)
BS2617
-2.559


hypothetical protein (yqaN)
BS0806
-2.555


acetoin dehydrogenase E1 component (TPP-dependent alpha subunit) (acoA)
BS0636
-2.553


GMP synthetase (guaA)
BS1871
-2.546


hypothetical protein (yoaS)
BS2152
-2.537


hypothetical protein (yolA)
BS1490
-2.535


cytochrome caa3 oxidase (subunit II) (ctaC)
BS3210
-2.518


transcriptional regulator (paiA)
BS0558
-2.516


hypothetical protein (ydgC)
BS1585
-2.516


similar to phosphoglycerate dehydrogenase (yloW)
BS3625
-2.513


transcriptional regulator (DeoR family) (glcR)
BS2512
-2.483


hypothetical protein (yqfN)
BS0164
-2.480


similar to transcriptional regulator (AraC/XylS family) (ybbB)
BS2895
-2.471


hypothetical protein (ytcF)
BS2632
-2.456


hypothetical protein (yrkS)
BS0804
-2.447


hypothetical protein (yfjM)
BS0632
-2.404


similar to cation efflux system membrane protein (yeaB)
BS2632
-2.392


hypotheitcal protein (yrkS)
BS2713
-2.387


similar to formate dehydrogenase (yrhG)
BS0561
-2.386


ATP-binding transport protein (expZ)
BS1373
-2.372


hypothetical protein (ykvJ)
BS0888
-2.371


hypothetical protein (ygaO)
BS2239
-2.369


ketopantoate hydroxymethyltransferase (panB)
BS2746
-2.356


hypothetical protein (yrvN)
BS3568
-2.347


UDP-glucose:polyglycerol phosphate glucosyltransferase (tagE)
BS4087
-2.342


similar to formate dehydrogenase (yyaE)
BS0745
-2.309


similar to quinone oxidoreductase (yfmJ)
BS3473
-2.308


similar to mutator MutT protein (yvcI)
BS2596
-2.308


similar to phage-related protein (yqbN)
BS1319
-2.296


cobalamin-independent methionine synthase (metC)
BS1472
-2.294


hypothetical protein (ylaA)
BS3265
-2.287


similar to ABC transporter (ATP-binding protein) (yurY)
BS3666
-2.250


required for formate dehydrogenase activity (narQ)
BS2225
-2.236


hypothetical protein (yppD)
BS3000
-2.232


hypothetical protein (ytfP)
BS1181
-2.230


hypothetical protein (yjcB)
BS1735
-2.220


hypothetical protein (ymzC)
BS0668
-2.210


hypothetical protein (yerL)
BS1065
-2.204


similar to DNA exonuclease (yirY)
BS3199
-2.201


hypothetical protein (yuiF)
BS1302
-2.182


hypothetical protein (ykgB)
BS2921
-2.181


hypothetical protein (ytoI)
BS3142
-2.171


similar to multidrug-efflux transporter (yuxJ)
BS2729
-2.163


similar to caffeoyl-CoA O-methyltransferase (yrrM)
BS0313
-2.157


hypothetical protein (ycgI)
BS3278
-2.157


similar to 3-hydroxyacyl-CoA dehydrogenase (yusL)
BS1386
-2.151


similar to heavy metal-transporting ATPase (ykvW)
BS0245
-2.139


similar to two-component sensor histidine kinase [YcbB] (ycbA)
BS3196
-2.124


hypothetical protein (yuiI)
BS0826
-2.115


similar to metabolite transport protein (yfiG)
BS1536
-2.115


similar to acetylornithine deacetylase (ylmB)
BS1142
-2.104


hypothetical protein (yjbA)
BS2376
-2.057


similar to pyrroline-5-carboxylate reductase (yqjO)
BS2920
-2.018


hypothetical protein (ytpI)
BS1878
-2.018


beta-lactamase precursor (penP)

First column is gene ID, second is Z score based on method in Materials and methods. Third column is checked if an attenuator has already been shown experimentally. Fourth column indicates if a promoter was predicted from NNPP program. Fifth column lists gene description. a. The NNPP program (cutoff = 0.8) was used for the upstream promoter prediction b GenBank annotation was used for gene definitions and gene names

Lathe et al. Genome Biology 2002 3:preprint0003.1   doi:10.1186/gb-2002-3-6-preprint0003