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Primers used for the generation and verification of yeast single knockouts |
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| Oligonucleotides for the synthesis of kanMX4 cassettes |
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| Primer |
Sequence 5' to 3' |
| YGR038w F |
AGG AAC TGC TGA GGC GGC TTC TAC CTC GTA TAG TCG AAA TCA AAC CGT ACG CTG CAG GTC GAC |
| YGR038w R |
TGT TCA ACT AAT TTG GGC GCG ACC TGT GAT ACC TGG GAT AGA AAT ATC GAT GAA TTC GAG CTC G |
| YLR350w F |
ATC ATG ATT GAC CGC ACT AAA AAC GAA TCT CCA GCT TTT GCG TAC GCT GCA GGT CGA C |
| YLR350w R |
CTA ACT AAT TTG AGC ACG GCC CGT AAT ACC AGG GAT GGA TAT CGA TGA ATT CGA GCT CG |
| Oligonucleotides for gene integrity and gene deletion verification |
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| Verification of ORM1 gene integrity |
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| A1 (F) |
GTT TAG GAA CGG ATT ATA GA |
| A2 (R) |
CTG GTC ATT TTC ATC TTC AA |
| Verification of ORM1 substitution |
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| A1 (F) |
GTT TAG GAA CGG ATT ATA GA |
| KAN5 (R) |
GTT CGG ATG TGA TGT GAG |
| Verification of ORM2 gene integrity |
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| YLR350w up400 (F) |
GCG TAT TTT GAT TGC TCA TA |
| YLR350w ver (R) |
TGG TTT CAG GTT AGA CAC AT |
| Verification of ORM2 substitution |
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| YLR350w up400 (F) |
GCG TAT TTT GAT TGC TCA TA |
| KAN5 (R) |
GTT CGG ATG TGA TGT GAG |
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Oligonucleotides in bold are 18-19 nucleotide homology regions to pFA6a MCS sequences. | |
Hjelmqvist et al. Genome Biology 2002 3:research0027.1 doi:10.1186/gb-2002-3-6-research0027 |
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