Table 1

Internal control genes evaluated in this study

Symbol

Accession number

Name

Function

Localization

Pseudo gene*

Primers

Alias

IMAGE


ACTB

NM_001101

Beta actin

Cytoskeletal structural protein

7p15-p12

+

S

510455

B2M

NM_004048

Beta-2-microglobulin

Beta-chain of major histocompatibility complex class I molecules

15q21-q22

-

S

51940

GAPD

NM_002046

Glyceraldehyde-3-phosphate dehydrogenase

Oxidoreductase in glycolysis and gluconeogenesis

12p13

+

D

510510

HMBS

NM_000190

Hydroxymethyl-bilane synthase

Heme synthesis, porphyrin metabolism

11q23

-

D

Porphobilinogen deaminase

245564

HPRT1

NM_000194

Hypoxanthine phosphoribosyl-transferase 1

Purine synthesis in salvage pathway

Xq26

+

D

345845

RPL13A

NM_012423

Ribosomal protein L13a

Structural component of the large 60S ribosomal subunit

19q13

+

D

23 kDa highly basic protein

-

SDHA

NM_004168

Succinate dehydrogenase complex, subunit A

Electron transporter in the TCA cycle and respiratory chain

5p15

+

D

375812

TBP

NM_003194

TATA box binding protein

General RNA polymerase II transcription factor

6q27

-

D

280735

UBC

M26880

Ubiquitin C

Protein degradation

12q24

-

D

510582

YWHAZ

NM_003406

Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, zeta polypeptide

Signal transduction by binding to phosphorylated serine residues on a variety of signaling molecules

2p25

+

S§

Phospholipase A2

416026


*Presence (+) or absence (-) of a retropseudogene in the genome determined by BLAST analysis of the mRNA sequence using the high-throughput genomic sequences database (htgs) or human genome as database. Localization of forward and reverse primer in different exons (D) or the same exon (S). IMAGE cDNA clone number according to [14]. § A single-exon gene.

Vandesompele et al. Genome Biology 2002 3:research0034.1-research0034.11   doi:10.1186/gb-2002-3-7-research0034

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