Table 1

Internal control genes evaluated in this study

Symbol

Accession number

Name

Function

Localization

Pseudo gene^*

Primers^†

Alias

IMAGE^‡

ACTB

NM_001101

Beta actin

Cytoskeletal structural protein

7p15-p12

+

S

510455

B2M

NM_004048

Beta-2-microglobulin

Beta-chain of major histocompatibility complex class I molecules

15q21-q22

-

S

51940

GAPD

NM_002046

Glyceraldehyde-3-phosphate dehydrogenase

Oxidoreductase in glycolysis and gluconeogenesis

12p13

+

D

510510

HMBS

NM_000190

Hydroxymethyl-bilane synthase

Heme synthesis, porphyrin metabolism

11q23

-

D

Porphobilinogen deaminase

245564

HPRT1

NM_000194

Hypoxanthine phosphoribosyl-transferase 1

Purine synthesis in salvage pathway

Xq26

+

D

345845

RPL13A

NM_012423

Ribosomal protein L13a

Structural component of the large 60S ribosomal subunit

19q13

+

D

23 kDa highly basic protein

-

SDHA

NM_004168

Succinate dehydrogenase complex, subunit A

Electron transporter in the TCA cycle and respiratory chain

5p15

+

D

375812

TBP

NM_003194

TATA box binding protein

General RNA polymerase II transcription factor

6q27

-

D

280735

UBC

M26880

Ubiquitin C

Protein degradation

12q24

-

D

510582

YWHAZ

NM_003406

Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, zeta polypeptide

Signal transduction by binding to phosphorylated serine residues on a variety of signaling molecules

2p25

+

S^§

Phospholipase A2

416026

^*Presence (+) or absence (-) of a retropseudogene in the genome determined by BLAST analysis of the mRNA sequence using the high-throughput genomic sequences database (htgs) or human genome as database. ^† Localization of forward and reverse primer in different exons (D) or the same exon (S). ^‡ IMAGE cDNA clone number according to [14]. ^§ A single-exon gene.

Vandesompele et al. Genome Biology 2002 3:research0034.1-research0034.11   doi:10.1186/gb-2002-3-7-research0034