Figure 3.

MEST markers substantially narrow the Dgt mapping region. Each bar shows the putative breakpoint of recombination in an informative BC₁ individual. Closed and open bars represent the chromosomal fraction of L. pennellii (wild-type phenotype) and L. esculentum (mutant phenotype), respectively. Mutant (M) or wild-type (W) phenotype is indicated for each individual plant and identifying number. On the basis of CAPS analysis, three MEST markers, BG643476, TC85079, and TC98260, were identified as new intervening markers between TG389 and the flanking RFLP markers, TG269 and CT190, with new recombination events identified in BC₁ individuals M710 and W505, respectively. TC89380, AW624844, and TC92082 co-segregated with TG389 and, thus are tightly linked to the Dgt gene. Genetic distances between molecular markers are indicated at the top.