Figure 3.

HAP4-overexpressing cells contain more mitochondrial structures with greater DASPMI fluorescence than wild-type cells. DASPMI fluorescence of glucose-grown wild-type and HAP4-overproducing cells was compared in microscopic preparations of mixed cells. To identify HAP4-overexpressing cells or wild-type cells in situ, microscopic preparations were analyzed in which only one of the strains was additionally stained with CalcoFluorWhite (CFW), a dye that stains the cell wall, in particular the bud-neck and bud scars [6]. (a) Top panels show a mixture of HAP4-expressing cells stained with CFW + DASPMI and wild-type cells stained with DASPMI only. Left, CFW fluorescence; right, phase contrast. (b) Top panels show a mixture of wild-type cells stained with CFW + DASPMI and HAP4-overexpressing cells stained with DASPMI only. Left, CFW fluorescence; right, phase contrast. In both (a) and (b) the large bottom panels show a mixture of wild-type and HAP4-overexpressing cells stained with DASPMI only.