Table 1

EST sequence summary of 25 positive R. flavipes clones identified from array screens

Clone *

Size (bp)

Identity match by BLASTx (%)

E-value

Gene ontogeny


1 (D04)

615

98% to Reticulitermes speratus salivary cellulase

1e-106

E

2 (C02)

423

89% to Reticulitermes speratus endoglucanase 2

1e-79

E

3 (E11)

505

57% to Pseudotrichonympha grassii cellulase

7e-69

E

4 (B02)

518

87% to Blattella germanica cytochrome oxidase I

7e-54

ET

5 (A10)

449

Unknown (65% to Anopheles gambiae unknown)

2e-49

UNK

6 (C07)

505

>65% to Drosophila melanogaster β-NAC homolog, 'bicaudal'

2e-45

TF

7 (B09)

585

69% to Holomastigotoides mirabile endoglucanase 2

1e-47

E

8 (C11)

509

69% to Drosophila melanogaster muscle-specific 'LIM' protein

2e-35

CSP

9 (B11)

569

80% to Drosophila melanogaster troponin I 'wings up'

1e-35

CSP

10 (H04)

242

98% to Bombyx mori β-tubulin

6e-33

CSP

11 (D10)

499

39% to Drosophila melanogaster troponin I 'wings apart', 'heldup'

3e-29

CSP

12 (F10)

527

36% to Athalia rosae vitellogenin

4e-20

SP

13 (D03)

387

>64% to Drosophila melanogaster 'BAB-I' (BTB/POZ domain containing protein)

3e-18

TF

14 (G12)

296

81% to Drosophila melanogaster mitochondrial-ribosomal protein S2

1e-17

MOB

15 (G03)

340

49% to Mus musculus SH3-domain kinase binding protein

5e-10

ST

16 (D02)

645

43% to Homo sapiens Rab3 GTPase-activating protein

2e-8

ST

17 (H08)

297

39% to Caenorhabtidis elegans RNA-dependent DNA polymerase (RTase)

0.001

DRF

18 (B10)

350

Unknown (40% to Drosophila melanogaster GC1077 gene product)

0.03

UNK

19 (A05)

630

38% to goldfish mitochondrion hypothetical 18K protein

0.12

MOB

20 (D07)

471

44% to Oncorhyncus mykiss cyclic GMP-gated channel

0.39

IC

21 (H06)

632

27% to Musca domestica nanos posterior determinant homolog

1.2

TF

22 (E12)

372

34% to Moraxella bovis endonuclease Mbo II

3.2

DRF

23 (G06)

370

Unknown (36% to Anopheles gambiae unknown)

>5

UNK

24 (A02)

510

Unknown

>5

UNK

25 (E03)

392

Unknown

>5

UNK


Clones are listed according to the significance of their identify-based BLASTx score E-values. * Clone number, with sequence identifier position shown in parentheses. See Additional data file 1 for sequence details. Gene ontogeny classification based on Adams et al. [13]: E (enzyme), CSP (cytoskeletal/structural protein), DRF (DNA replication factor), ET (electron transport), IC (ion channel), MOB (mitochondrial organization and biogenesis), SP (storage protein), ST (signal transduction), TF (transcription factor), UNK (unknown).

Scharf et al. Genome Biology 2003 4:R62   doi:10.1186/gb-2003-4-10-r62

Open Data