Table 1 |
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|
EST sequence summary of 25 positive R. flavipes clones identified from array screens |
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| Clone * |
Size (bp) |
Identity match by BLASTx (%) |
E-value |
Gene ontogeny† |
|
|
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| 1 (D04) |
615 |
98% to Reticulitermes speratus salivary cellulase |
1e-106 |
E |
| 2 (C02) |
423 |
89% to Reticulitermes speratus endoglucanase 2 |
1e-79 |
E |
| 3 (E11) |
505 |
57% to Pseudotrichonympha grassii cellulase |
7e-69 |
E |
| 4 (B02) |
518 |
87% to Blattella germanica cytochrome oxidase I |
7e-54 |
ET |
| 5 (A10) |
449 |
Unknown (65% to Anopheles gambiae unknown) |
2e-49 |
UNK |
| 6 (C07) |
505 |
>65% to Drosophila melanogaster β-NAC homolog, 'bicaudal' |
2e-45 |
TF |
| 7 (B09) |
585 |
69% to Holomastigotoides mirabile endoglucanase 2 |
1e-47 |
E |
| 8 (C11) |
509 |
69% to Drosophila melanogaster muscle-specific 'LIM' protein |
2e-35 |
CSP |
| 9 (B11) |
569 |
80% to Drosophila melanogaster troponin I 'wings up' |
1e-35 |
CSP |
| 10 (H04) |
242 |
98% to Bombyx mori β-tubulin |
6e-33 |
CSP |
| 11 (D10) |
499 |
39% to Drosophila melanogaster troponin I 'wings apart', 'heldup' |
3e-29 |
CSP |
| 12 (F10) |
527 |
36% to Athalia rosae vitellogenin |
4e-20 |
SP |
| 13 (D03) |
387 |
>64% to Drosophila melanogaster 'BAB-I' (BTB/POZ domain containing protein) |
3e-18 |
TF |
| 14 (G12) |
296 |
81% to Drosophila melanogaster mitochondrial-ribosomal protein S2 |
1e-17 |
MOB |
| 15 (G03) |
340 |
49% to Mus musculus SH3-domain kinase binding protein |
5e-10 |
ST |
| 16 (D02) |
645 |
43% to Homo sapiens Rab3 GTPase-activating protein |
2e-8 |
ST |
| 17 (H08) |
297 |
39% to Caenorhabtidis elegans RNA-dependent DNA polymerase (RTase) |
0.001 |
DRF |
| 18 (B10) |
350 |
Unknown (40% to Drosophila melanogaster GC1077 gene product) |
0.03 |
UNK |
| 19 (A05) |
630 |
38% to goldfish mitochondrion hypothetical 18K protein |
0.12 |
MOB |
| 20 (D07) |
471 |
44% to Oncorhyncus mykiss cyclic GMP-gated channel |
0.39 |
IC |
| 21 (H06) |
632 |
27% to Musca domestica nanos posterior determinant homolog |
1.2 |
TF |
| 22 (E12) |
372 |
34% to Moraxella bovis endonuclease Mbo II |
3.2 |
DRF |
| 23 (G06) |
370 |
Unknown (36% to Anopheles gambiae unknown) |
>5 |
UNK |
| 24 (A02) |
510 |
Unknown |
>5 |
UNK |
| 25 (E03) |
392 |
Unknown |
>5 |
UNK |
|
|
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|
Clones are listed according to the significance of their identify-based BLASTx score E-values. * Clone number, with sequence identifier position shown in parentheses. See Additional data file 1 for sequence details. †Gene ontogeny classification based on Adams et al. [13]: E (enzyme), CSP (cytoskeletal/structural protein), DRF (DNA replication factor), ET (electron transport), IC (ion channel), MOB (mitochondrial organization and biogenesis), SP (storage protein), ST (signal transduction), TF (transcription factor), UNK (unknown). |
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|
Scharf et al. Genome Biology 2003 4:R62 doi:10.1186/gb-2003-4-10-r62 |
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