Table 2

Relative expression levels of R. flavipes gene transcripts


Caste
 Developmental stage


Gene ontogeny category and clone identity
 Accession number‡
 Worker
 Soldier
 Presoldier
 Nymph
Standard/control




β-actin†
 N/A (N/A)
 1.1 ± 0.1
 1.0 ± 0.4
 1.0 ± 0.1
 1.1 ± 0.1






Transcription/translation factor




6. Rf β-NAC-1, 'bicaudal'
 AY258589
 5.2 ± 0.2
 13.8 ± 0.3
 1.4 ± 0.1
 1.0 ± 0.1
21. Rf PDL, 'nanos'-like
 BQ788190
 3.0 ± 0.0
 5.0 ± 0.1
 1.0 ± 0.5
 2.2 ± 0.1
13. Rf BTB/POZ-1, 'BAB'
 AY258590
 2.2 ± 0.3
 1.7 ± 0.0
 1.0 ± 0.1
 2.6 ± 0.2






Signal transduction




15. SH3-domain kinase-binding
 CB518313
 85.5 ± 0.1
 131.9 ± 0.2
 1.0 ± 0.2
 3.6 ± 1.3
16. GTPase-activating protein
 BQ788178
 2.3 ± 0.3
 4.5 ± 0.1
 1.0 ± 0.4
 1.3 ± 0.0






Enzyme




1. Termite salivary cellulase
 CB518295
 31.5 ± 0.1
 1.6 ± 0.0
 1.0 ± 0.0
 19.5 ± 0.0
2. Termite endoglucanase 2
 CB518296
 32.3 ± 4.8
 2.5 ± 1.5
 1.0 ± 0.1
 30.1 ± 1.1
3. Symbiont cellulase
 CB518297
 1939.7 ± 0.0
 1.7 ± 0.2
 1.0 ± 0.4
 208.9 ± 0.2
7. Symbiont endoglucanase 2
 CB518298
 62.7 ± 0.9
 2.7 ± 1.1
 1.0 ± 0.6
 34.4 ± 0.1






Cytoskeletal/structural protein




8. Muscle LIM protein
 CB518301
 1.6 ± 0.3
 5.0 ± 0.2
 1.8 ± 0.3
 1.0 ± 1.1
9. Rf1-troponin I
 CB518302
 2.3 ± 0.1
 3.9 ± 0.4
 1.4 ± 0.2
 1.0 ± 0.0
10. β-tubulin
 CB518304
 1.6 ± 0.3
 5.0 ± 0.2
 1.8 ± 0.3
 1.0 ± 1.1
11. Rf2-troponin I
 CB518303
 1.1 ± 1.3
 11.5 ± 0.6
 1.0 ± 1.2
 1.9 ± 3.0






Storage/hormone binding




12. Vitellogenin
 CB518311
 1.2 ± 0.6
 1.0 ± 0.1
 4.9 ± 0.2
 1.4 ± 1.7






Electron transport




4. Cytochrome oxidase I
 CB518306
 3.1 ± 0.6
 6.6 ± 0.1
 2.5 ± 0.2
 1.0 ± 0.0






Mitochondrial organization/biogenesis




14. mRpS2
 CB518308
 4.3 ± 0.0
 4.0 ± 0.0
 1.0 ± 0.7
 1.9 ± 0.1
19. 18K protein-like
 CB518307
 2.9 ± 0.0
 1.3 ± 0.9
 1.0 ± 0.1
 2.3 ± 1.5






DNA replication factor




17. Reverse transcriptase-like
 CB518309
 1.4 ± 1.5
 2.1 ± 0.7
 1.2 ± 0.1
 1.0 ± 0.7
22. MboII endonuclease-like
 CB518310
 1.1 ± 1.3
 5.3 ± 0.3
 2.8 ± 0.1
 2.2 ± 0.4






Unknown




5. Unknown
 CB518314
 2.2 ± 0.8
 6.1 ± 0.4
 1.0 ± 0.9
 2.6 ± 0.1
18. Unknown
 CB518315
 9.5 ± 0.2
 1.2 ± 0.5
 1.1 ± 0.1
 1.0 ± 0.3
23. Unknown
 CB518318
 1.0 ± 0.2
 5.9 ± 0.2
 5.4 ± 2.5
 1.2 ± 2.0
24. Unknown
 CB518317
 12.4 ± 0.1
 2.3 ± 0.1
 1.0 ± 0.0
 1.9 ± 0.1
25. Unknown
 CB518319
 1.1 ± 0.1
 2.1 ± 0.2
 1.5 ± 0.2
 1.0 ± 0.5

Relative expression levels of R. flavipes gene transcripts (average ± standard error), identified using cDNA macroarrays and quantified by quantitative PCR. Data shown in bold-face type represent the highest relative expression for a given gene (LSD t-test; P ≤ 0.05). Relative expression calculated using the method described by Pfaffl et al. [44] (n = 3). See Additional data file 2 for raw data. †All results are normalized to the control gene β-actin. Primers designed from mouse and rat sequences (Accession nos. X03672 and V01207), as described by Giulietti et al. [43]. ‡Clone numbers correspond to the order shown in Table 1 (numbers 16 and lower have significant identity scores). See Additional data file 1 and 9, respectively, for sequence and primer details.

Scharf et al. Genome Biology 2003 4:R62   doi:10.1186/gb-2003-4-10-r62

Open Data