Table 2

Relative expression levels of R. flavipes gene transcripts

Caste

Developmental stage



Gene ontogeny category and clone identity

Accession number

Worker

Soldier

Presoldier

Nymph


Standard/control

β-actin

N/A (N/A)

1.1 ± 0.1

1.0 ± 0.4

1.0 ± 0.1

1.1 ± 0.1

Transcription/translation factor

6. Rf β-NAC-1, 'bicaudal'

AY258589

5.2 ± 0.2

13.8 ± 0.3

1.4 ± 0.1

1.0 ± 0.1

21. Rf PDL, 'nanos'-like

BQ788190

3.0 ± 0.0

5.0 ± 0.1

1.0 ± 0.5

2.2 ± 0.1

13. Rf BTB/POZ-1, 'BAB'

AY258590

2.2 ± 0.3

1.7 ± 0.0

1.0 ± 0.1

2.6 ± 0.2

Signal transduction

15. SH3-domain kinase-binding

CB518313

85.5 ± 0.1

131.9 ± 0.2

1.0 ± 0.2

3.6 ± 1.3

16. GTPase-activating protein

BQ788178

2.3 ± 0.3

4.5 ± 0.1

1.0 ± 0.4

1.3 ± 0.0

Enzyme

1. Termite salivary cellulase

CB518295

31.5 ± 0.1

1.6 ± 0.0

1.0 ± 0.0

19.5 ± 0.0

2. Termite endoglucanase 2

CB518296

32.3 ± 4.8

2.5 ± 1.5

1.0 ± 0.1

30.1 ± 1.1

3. Symbiont cellulase

CB518297

1939.7 ± 0.0

1.7 ± 0.2

1.0 ± 0.4

208.9 ± 0.2

7. Symbiont endoglucanase 2

CB518298

62.7 ± 0.9

2.7 ± 1.1

1.0 ± 0.6

34.4 ± 0.1

Cytoskeletal/structural protein

8. Muscle LIM protein

CB518301

1.6 ± 0.3

5.0 ± 0.2

1.8 ± 0.3

1.0 ± 1.1

9. Rf1-troponin I

CB518302

2.3 ± 0.1

3.9 ± 0.4

1.4 ± 0.2

1.0 ± 0.0

10. β-tubulin

CB518304

1.6 ± 0.3

5.0 ± 0.2

1.8 ± 0.3

1.0 ± 1.1

11. Rf2-troponin I

CB518303

1.1 ± 1.3

11.5 ± 0.6

1.0 ± 1.2

1.9 ± 3.0

Storage/hormone binding

12. Vitellogenin

CB518311

1.2 ± 0.6

1.0 ± 0.1

4.9 ± 0.2

1.4 ± 1.7

Electron transport

4. Cytochrome oxidase I

CB518306

3.1 ± 0.6

6.6 ± 0.1

2.5 ± 0.2

1.0 ± 0.0

Mitochondrial organization/biogenesis

14. mRpS2

CB518308

4.3 ± 0.0

4.0 ± 0.0

1.0 ± 0.7

1.9 ± 0.1

19. 18K protein-like

CB518307

2.9 ± 0.0

1.3 ± 0.9

1.0 ± 0.1

2.3 ± 1.5

DNA replication factor

17. Reverse transcriptase-like

CB518309

1.4 ± 1.5

2.1 ± 0.7

1.2 ± 0.1

1.0 ± 0.7

22. MboII endonuclease-like

CB518310

1.1 ± 1.3

5.3 ± 0.3

2.8 ± 0.1

2.2 ± 0.4

Unknown

5. Unknown

CB518314

2.2 ± 0.8

6.1 ± 0.4

1.0 ± 0.9

2.6 ± 0.1

18. Unknown

CB518315

9.5 ± 0.2

1.2 ± 0.5

1.1 ± 0.1

1.0 ± 0.3

23. Unknown

CB518318

1.0 ± 0.2

5.9 ± 0.2

5.4 ± 2.5

1.2 ± 2.0

24. Unknown

CB518317

12.4 ± 0.1

2.3 ± 0.1

1.0 ± 0.0

1.9 ± 0.1

25. Unknown

CB518319

1.1 ± 0.1

2.1 ± 0.2

1.5 ± 0.2

1.0 ± 0.5


Relative expression levels of R. flavipes gene transcripts (average ± standard error), identified using cDNA macroarrays and quantified by quantitative PCR. Data shown in bold-face type represent the highest relative expression for a given gene (LSD t-test; P ≤ 0.05). Relative expression calculated using the method described by Pfaffl et al. [44] (n = 3). See Additional data file 2 for raw data. All results are normalized to the control gene β-actin. Primers designed from mouse and rat sequences (Accession nos. X03672 and V01207), as described by Giulietti et al. [43]. Clone numbers correspond to the order shown in Table 1 (numbers 16 and lower have significant identity scores). See Additional data file 1 and 9, respectively, for sequence and primer details.

Scharf et al. Genome Biology 2003 4:R62   doi:10.1186/gb-2003-4-10-r62

Open Data