Discrimination between present and absent exon-exon junctions in RB1 mRNA. Each line shows the intensities of a series of 40-nucleotide probes starting entirely within exon 9 and, at one-nucleotide steps, tiling through the junction between exon 9 and each of exons 10, 11, 12, and 13. Probes on the left side of the plot should hybridize completely within exon 9 and have similar intensities, whereas probes on the far right side (entirely within exons 10, 11, 12, or 13) have intensities that differ because of variations in exon sequence composition. Probes in the central section of the figure are chimeric sequences complementary to portions of two different exons. Probes between non-connected exons (for example, exon 9 and 13) have lower intensities whereas the probes between connected exons (exon 9 and 10) remain at high intensities. Background level is shown by the black dashed line at the bottom of the figure.
Castle et al. Genome Biology 2003 4:R66 doi:10.1186/gb-2003-4-10-r66