Detection of isoform mixtures in a single sample, using purified ANXA7 RT-PCR products from skeletal (Sk) and smooth (Sm) muscle and 60-nucleotide probes. Both isoforms can be detected when the shorter isoform, without exon 6, makes up 20% or more of the mixture. Plotted are log-ratios between intensities after normalizing on the basis of intensities of probes within unaffected exons. Blue and green lines trace exon and junction probe intensities, respectively; numbers are exon numbers. This experiment was performed for mixtures of 0, 20, 40, 60, 80, and 100% smooth muscle (see Additional data file 3).
Castle et al. Genome Biology 2003 4:R66 doi:10.1186/gb-2003-4-10-r66