Table 1

Optimal probe lengths, probe positions, and formamide concentrations for purposes of exon monitoring, exon edge detection, and exon-exon junction monitoring

Exon monitoring

Exon-edge detection

Junction monitoring

Probe size

50 to 60 nucleotides

35 nucleotides

35 to 40 nucleotides

Probe position

Exon interior

= 5-nucleotide steps

Centered ± 5 nucleotides

Formamide concentration

35%

30%

30 to 40%

Castle et al. Genome Biology 2003 4:R66   doi:10.1186/gb-2003-4-10-r66