Table 2

Summary of cDNA screen for each library and probe.

Library

Probe

Number of plaques screened (× 103)

Number of sequences obtained

Number of real olfactory receptor sequences

True-positive rate

Olfactory receptor clone frequency

Number of olfactory receptor genes represented


Embryonic

OR5B_OR3B_40

640

58

37

64%

1/17,300

27

Adult

OR5B_OR3B_40

2,850

1,450

1,138

78%

1/2,500

394

P24_P28_40 and TM3deg1_P28_45

200

23

3

13%

1/66,700

3

P26_P27_45

700

135

58

43%

1/12,100

35

P24_P28_45

200

39

22

56%

1/9,100

19

OR5B_OR3B_45

150

10

6

60%

1/250,000

5

Total

4,740

1,715

1,264

74%

1/3,800

419


Probe names comprise the names of the two primers and the annealing temperature used during PCR to generate the probes, separated by underscores

Young et al. Genome Biology 2003 4:R71   doi:10.1186/gb-2003-4-11-r71

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