Figure 4.

A Venn diagram reveals shared gene sets between cycad contigs versus lower plants, gymnosperms and/or angiosperms. BLASTX (cut-off E value > 1e-5) was used to compare the cycad contigs against all angiosperm ESTs and annotated genes from the full Arabidopsis and rice genome sequence from GenBank. Genes that do not have a match to angiosperm genes were then compared to available ESTs from all gymnosperms or lower-plant ESTs available in GenBank. Genes that are common to cycads and more than one group are shown in the intersecting (shaded) regions.